FIGURE

Fig. 8

ID

ZDB-FIG-150615-8

Publication

Krock et al., 2014 - The Par-PrkC Polarity Complex Is Required for Cilia Growth in Zebrafish Photoreceptors

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Fig. 8

Photo-Morpholinos reduce apical domain size. (A–D) Transverse cryosections of 4 dpf retinas were stained with phalloidin to label actin (red) and zpr-1 to label double cones (green). Sections were also counterstained with DAPI (blue). (E) Quantification of apical domain as a percentage of total photoreceptor inner segment length. All values are plotted as a percentage of n/m, as defined in the text. Wild type = 38%; pard3-pard3pMO morphants = 16%; prkci-prkcipMO morphants = 9.72%; prkc-prkcpMO was non-detectable. Blue error bars indicate the mean ± standard deviation. ONL = outer nuclear layer; OPL = outer plexiform layer; INL = inner nuclear layer. Scale bar = 20 μm.

Expression Data

Antibody:	zpr-1
Fish:	AB/EKW AB/EKW + MO1-pard3ab AB/EKW + MO1-prkci AB/EKW + MO1-prkci + MO1-prkcz
Knockdown Reagents:	MO1-pard3ab MO1-prkci MO1-prkcz
Anatomical Term:	retinal cone cell
Stage:	Day 4

Expression Detail

No data available

Antibody Labeling

Phenotype Data

Fish:	AB/EKW + MO1-pard3ab AB/EKW + MO1-prkci AB/EKW + MO1-prkci + MO1-prkcz
Knockdown Reagents:	MO1-pard3ab MO1-prkci MO1-prkcz
Observed In:	camera-type eye photoreceptor cell differentiation eye photoreceptor cell apical region photoreceptor cell morphogenesis retina retinal cone cell
Stage:	Day 4

Phenotype Detail

Fish	Conditions	Stage	Phenotype
AB/EKW + MO1-pard3ab	standard conditions	Day 4	eye photoreceptor cell apical region decreased length, abnormal
AB/EKW + MO1-pard3ab	standard conditions	Day 4	photoreceptor cell morphogenesis decreased process quality, abnormal
AB/EKW + MO1-prkci	standard conditions	Day 4	eye photoreceptor cell apical region decreased length, abnormal
AB/EKW + MO1-prkci	standard conditions	Day 4	photoreceptor cell morphogenesis decreased process quality, abnormal
AB/EKW + MO1-prkci + MO1-prkcz	standard conditions	Day 4	camera-type eye photoreceptor cell differentiation arrested, abnormal
AB/EKW + MO1-prkci + MO1-prkcz	standard conditions	Day 4	retina lacks all parts of type retinal cone cell, abnormal

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Acknowledgments

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