FIGURE

Fig. S3

ID

ZDB-FIG-150422-67

Publication

Olena et al., 2015 - miR-216a regulates snx5, a novel notch signaling pathway component, during zebrafish retinal development

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Fig. S3

Knockdown of snx5 and overexpression of miR-216a result in vascular patterning defects. Tg(flk1:eGFP) embryos were injected at the 1-cell stage with dye control, snx5^MOs, or miR-216a. Embryos were grown in egg water and vascular development was monitored. Intersegmental vessels were missing in the trunks of snx5^MOs - and miR-216a-injected larvae at 4dpf, but not from dye control-injected larvae. Left column contains representative images, and right column shows vessels outlined with dotted white lines in order to highlight the absence of some vessels.

Expression Data

Gene:	GFP
Fish:	la116Tg la116Tg + MO1-snx5 + MO2-snx5
Knockdown Reagents:	MO1-snx5 MO2-snx5
Anatomical Terms:	intersegmental vessel trunk vasculature
Stage:	Day 4

Expression Detail

Gene	Fish	Conditions	Stage	Anatomy	Assay
GFP	la116Tg	control	Day 4	intersegmental vessel	IFL
GFP	la116Tg	control	Day 4	trunk vasculature	IFL
GFP	la116Tg + MO1-snx5 + MO2-snx5	standard conditions	Day 4	intersegmental vessel	IFL
GFP	la116Tg + MO1-snx5 + MO2-snx5	standard conditions	Day 4	trunk vasculature	IFL

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Antibody Labeling

Phenotype Data

Fish:	la116Tg + MO1-snx5 + MO2-snx5
Knockdown Reagents:	MO1-snx5 MO2-snx5
Observed In:	intersegmental vessel trunk vasculature blood vessel morphogenesis
Stage:	Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 400(1), Olena, A.F., Rao, M.B., Thatcher, E.J., Wu, S.Y., Patton, J.G., miR-216a regulates snx5, a novel notch signaling pathway component, during zebrafish retinal development, 72-81, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.