FIGURE

Fig. 2

ID

ZDB-FIG-141007-45

Publication

Bielczyk-Maczyńska et al., 2014 - A loss of function screen of identified genome-wide association study Loci reveals new genes controlling hematopoiesis

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Fig. 2

Characterization of HSCs in candidate gene depleted embryos.

To assess the stage at which hematopoiesis of each MO injected embryo was defective, we performed whole mount in situ hybridization using a c-myb probe at 3 dpf. Although more than half of MOs had no effect on the number of HSCs, depletion of rcor1 resulted in increased numbers of HSCs and depletion of kalrn1, kalrn2, mfn2, pdia5, psmd13 and wasplb resulted in decreased numbers of HSCs in CHT at 3 dpf. Representative images of CHT region are shown. All embryos are oriented with anterior to the left and dorsal to the top.

Expression Data

Gene:	myb
Fish:	WT WT + MO1-akap10 WT + MO1-brd3a WT + MO1-brf1a WT + MO1-brf1b WT + MO1-cfap251 WT + MO1-kalrna WT + MO1-kalrnb WT + MO1-pdia5 WT + MO1-psmd13 WT + MO1-satb1b WT + MO1-wasla WT + MO1-waslb WT + MO2-mfn2 WT + MO2-rcor1 WT + MO4-kif1b (all 16)
Knockdown Reagents:	MO1-akap10 MO1-brd3a MO1-brf1a MO1-brf1b MO1-cfap251 MO1-kalrna MO1-kalrnb MO1-pdia5 MO1-psmd13 MO1-satb1b MO1-wasla MO1-waslb MO2-mfn2 MO2-rcor1 MO4-kif1b (all 15)
Anatomical Term:	hematopoietic stem cell
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-kalrna WT + MO1-kalrnb WT + MO1-pdia5 WT + MO1-psmd13 WT + MO1-waslb WT + MO2-mfn2 WT + MO2-rcor1 (all 7)
Knockdown Reagents:	MO1-kalrna MO1-kalrnb MO1-pdia5 MO1-psmd13 MO1-waslb MO2-mfn2 MO2-rcor1 (all 7)
Observed In:	blood island hematopoietic stem cell
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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