FIGURE

Fig. 2

ID

ZDB-FIG-140117-18

Publication

Nagashima et al., 2013 - A self-renewing division of zebrafish Muller glial cells generates neuronal progenitors that require N-cadherin to regenerate retinal neurons

Other Figures

(all 13)

All Figure Page

Back to All Figure Page

Fig. 2

Müller glia partially dedifferentiate and express retinal progenitor markers in response to photoreceptor lesions. (A-D) PCNA (green), BLBP (cyan) and Rx1 (white/magenta) in control (ctrl) retina. Higher magnifications of the boxed regions are shown on the right. (A) Cones are Rx1⁺ (asterisks, UV cone nuclei). (B) By 1 hpl, Rx1 is reduced in UV cones and BLBP is upregulated in Müller glia. (C) At 8 hpl, cones collapse (asterisk) and Müller glia express BLBP and Rx1 (boxed region). A PCNA⁺ microglia or rod precursor (arrowhead) can be seen in the ONL. (D) At 1 dpl, Müller glia are weakly PCNA⁺/BLBP⁺/Rx1⁺. (E,F) Injury-induced nGFP in lesioned area (asterisks) at 4 and 8 hpl and in immature Müller glia (arrowheads). Zpr1 (magenta), which recognizes Arrestin 3a and specifically labels red/green cones. Higher magnifications of the boxed regions are shown in insets. (C) Reduced rlbp1a transcript (white) by 4 hpl and no detectable signal at 1 dpl. Scale bars: 10 μm (A-D,G); 100 μm (E,F).

Expression Data

Genes:	arr3a EGFP rlbp1a
Antibodies:	Ab1-rx1 Ab3-fabp7 zpr-1
Fish:	WT mi2004Tg
Condition:	light ablation: photoreceptor cell
Anatomical Terms:	Muller cell Muller cell nucleus retinal cone cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT mi2004Tg
Condition:	light ablation: photoreceptor cell
Observed In:	eye photoreceptor cell Muller cell retina retina sensory epithelium regeneration
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development