FIGURE

Fig. 1

ID

ZDB-FIG-131213-57

Publication

Dohn et al., 2013 - Planar cell polarity proteins differentially regulate extracellular matrix organization and assembly during zebrafish gastrulation

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Fig. 1

Knockdown of vangl2 and prickle1a decreases fibronectin. (A–C) Top panels show morphological convergence and extension phenotypes at tailbud stage (see arrowheads) of wild type, vangl2 morphant, and prickle1a morphant embryos. Middle/bottom panels show confocal images of fibronectin (Fn) immunolabeling without and with nuclear DAPI staining. Scale bars=20 μm. (D and E) Fn and GAPDH western blots of 10 μg total protein extract obtained from tailbud stage wild type, vangl2 mutant, glypican4 mutant, and prickle1a morphant embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-prickle1a WT + MO4-vangl2
Knockdown Reagents:	MO1-prickle1a MO4-vangl2
Observed In:	convergent extension
Stage:	Bud

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 383(1), Dohn, M.R., Mundell, N.A., Sawyer, L.M., Dunlap, J.A., and Jessen, J.R., Planar cell polarity proteins differentially regulate extracellular matrix organization and assembly during zebrafish gastrulation, 39-51, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.