FIGURE

Fig. 3

ID

ZDB-FIG-130801-3

Publication

Teslaa et al., 2013 - Zebrafish Zic2a and Zic2b regulate neural crest and craniofacial development

Other Figures

(all 15)

All Figure Page

Back to All Figure Page

Fig. 3

Zic2a regulates patterning of anterior chondrogenic condensations. 2 dpf zic2a morphants (A–F) and overexpressors (G,H) stained by ISH for col2a1a or sox9a. (A,B) Sox9a expression is reduced in the posterior PAs of Zic2a-depleted embryos (18/20, 1 exp.). (C,D) Zic2a morphants have shortened trabeculae (18/20, 1 exp.) and lack the medial ethmoid plate (12/20, 1 exp., arrow in 3C). (E,F) Expression of col2a1a reiterates the shortened trabeculae and lack of ethmoid plate in Zic2a-depleted embryos (43/43, 2 exp). (G,H) Col2a1a expression in wild-type siblings shows the bilateral trabeculae and ethmoid plate (67/107, 2 exp.). (H) The ethmoid plate is ablated and trabeculae are shortened by Zic2aYFP misexpression induced at 10 hpf (38/67, 2 exp). (A,B) are lateral views. (C–H) are ventral views, anterior to the left.

Expression Data

Genes:	col2a1a sox9a
Fish:	WT WT + MO3-zic2a uw6Tg; vu22Tg
Condition:	heat shock
Knockdown Reagent:	MO3-zic2a
Anatomical Terms:	ethmoid cartilage neurocranial trabecula neurocranium pharyngeal arch pharyngeal arch 1 pharyngeal arch 2 (all 6)
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-zic2a uw6Tg; vu22Tg
Condition:	heat shock
Knockdown Reagent:	MO3-zic2a
Observed In:	embryonic neurocranium morphogenesis embryonic viscerocranium morphogenesis ethmoid cartilage ethmoid cartilage medial region neurocranial trabecula pharyngeal arch (all 6)
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 380(1), Teslaa, J.J., Keller, A.N., Nyholm, M.K., and Grinblat, Y., Zebrafish Zic2a and Zic2b regulate neural crest and craniofacial development, 73-86, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.