Fig. S1

Characterization of reconstituted c-myb^-/- mutants. (A) Side views of wild-type, c-myb^-/- transplant recipients and a c-myb^-/- mutant fish. Note the absence of cardiac edema (resulting in a bulged body curvature) in the transplanted fish; the body curvature is highlighted by dotted lines. (Scale bar, 1 mm.) (B) Reconstitution of T-cell development in the thymus of c-myb^-/- mutant recipients transplanted with ikaros:eGFP-transgenic wild-type whole kidney marrow (WKM) cells; note the presence of green fluorescent cells in the transplanted fish. Photographs (side views) were taken 20 d after transplantation. (Scale bar, 50 μm.) (C and D) Whole-mount RNA in situ hybridization with c-myb-, mpx-, and rag-specific probes on thymus (C) and kidney (D) sections of wild-type, c-myb^-/- mutant and c-myb^-/- transplant recipients (3 wk after transplantation). In mutant tissues, no positive cells were observed for c-myb, mpx, and rag1, while expression patterns in transplanted c-myb^-/- recipients are indistinguishable from those of their wild-type siblings. c-myb is a general marker for hematopoietic cells; mpx is a marker for myeloid cells; rag1 is a marker for developing lymphocytes. (Scale bar, 50 μm.)