FIGURE

Fig. 5

ID

ZDB-FIG-120228-3

Publication

Stemple et al., 1996 - Mutations affecting development of the notochord in zebrafish

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Fig. 5

col2a1, shh, and Engrailed expression in mutants in the sly, gup, bal, dop, sny, and gno loci. Shown are DIC micrographs of (A-C) wildtype embryos, and (D-F) sly^m466, (G-I) gup^m189, (J-L) bal^m190, (M-O) dop^m341, (P-R) sny^m456, and (S-U) gno^m622 mutant embryos stained by in situ hybridization with anti-sense zebrafish col2a1 (A,D,G,J,M,P,S), shh (B,E,H,K,N,Q,T) RNA or with monoclonal anti-Engrailed antibody 4D9 (C,F,I,L,O,R,U). Embryos stained for col2a1 are at 32 hpf and embryos stained for shh or Engrailed are at 24 hpf. Scale bars, 250 μm.

Expression Data

Genes:	col2a1a shha
Antibody:	Ab1-eng
Fish:	AB copa^m456/m456 (AB) copb2^m341/m341 (AB) gno^m622/m622 (AB) lama1^m190/m190 (AB) lamb1a^m189/m189 (AB) lamc1^m466/m466 (AB) (all 7)
Anatomical Terms:	horizontal myoseptum muscle pioneer muscle pioneer notochord
Stage Range:	Prim-5 to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	copa^m456/m456 (AB) copb2^m341/m341 (AB) gno^m622/m622 (AB) lama1^m190/m190 (AB) lamb1a^m189/m189 (AB) lamc1^m466/m466 (AB) (all 6)
Observed In:	hypochord notochord notochord anterior region somite somite muscle pioneer
Stage Range:	Prim-5 to Prim-15

Phenotype Detail

Acknowledgments

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