Fig. 9
- ID
- ZDB-FIG-120228-15
- Publication
- Otten et al., 2012 - Xirp proteins mark injured skeletal muscle in zebrafish
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Efficient Morpholino antisense oligonucleotide-mediated knock-down of xirp2a does not affect myofibrillogenesis. (A) Phenotypically, xirp2a morphants are indistinguishable from WT embryos at 24 hpf. Scale bar: 250 μm. (B) Efficient gene knock-down of xirp2a is assessed by immunohistochemistry at 24 hpf. Green: Xirp2a; red: Actin. Scale bar: 20 μm. (C) Complete loss of Xirp2a does not affect myofibrillogenesis and correct sarcomeric organization of skeletal muscle as determined by immunohistochemistry using an antibody against the A/I junction epitope of Titin and rhodamine phalloidin to label sarcomeric Actin at 24 hpf. Green: Titin; red: Actin. Scale bar: 5 μm. |
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Stage: | Prim-5 |