Fig. 6

Loss of capillary blood flow in ponzr1 morphants. (A,C,E) Tg(wt1b:EGFP) in the podocytes was used to identify the glomerulus in a 3 dpf larvae. (B,D,F) Tg(gata1:dsRed) was used to visualize the blood cells flowing through the glomerular capillaries. (B) Wild-type larvae on the same plane as A show gata1-positive blood cells in the glomerular capillaries (arrows; see supplementary material Movie 1). The bright red line of cells running through the middle of the larvae is the dorsal aorta (arrowhead). (D) Mismatch MO-injected larvae also have blood cells flowing through the glomerular capillaries (arrows; see supplementary material Movie 2) and through the aorta (arrowhead). (F) ponzr1 morphants have no blood cells in the glomerular capillaries, but the cells flowing through the aorta can still be seen (arrowhead; see supplementary material Movie 3). (G) This phenotype is significantly different compared with both wild-type and mismatch MO-injected larvae (^*** P<0.001).