Fig. S1
- ID
- ZDB-FIG-111017-9
- Publication
- Ramachandran et al., 2011 - Ascl1a/Dkk/ beta -catenin signaling pathway is necessary and glycogen synthase kinase-3 beta inhibition is sufficient for zebrafish retina regeneration
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FACS and Wnt component gene expression. (A) Representative scatter plots used to FACS purify GFP-expressing cells. Wild type (Wt) retinal cells do not fall into the P4 window, whereas GFP+ retinal cells from gfap:gfp and 1016 tuba1a:gfp transgenic fish retinas readily sort into this window. (B) RT-PCR analysis of injury-dependent Wnt signaling component gene expression using total retina RNA or RNA from FACS-purified MG and MG-derived progenitors. Shown are Wnt signaling component genes that do not exhibit injury-dependent regulation in purified MG. |