Fig. S3
- ID
- ZDB-FIG-110408-7
- Publication
- Corti et al., 2011 - Interaction between alk1 and blood flow in the development of arteriovenous malformations
- Other Figures
- (all 9)
- All Figure Page
- Back to All Figure Page
Endothelial cell counts in fixed embryos are comparable with live embryos. Endothelial cells in the BCA/PCS were counted in five live Tg(fli1a:nEGFP)y7 tnnt2a morphants at 48 hpf, then counted again after fixation. Results revealed no difference in cell counts, with an average (mean±s.e.m.) of 41.2±1.35 cells in live embryos versus 40±1.09 cells in fixed embryos. It is difficult to count cells in collapsed vessels, so these images represent the greatest counting challenge. Two-dimensional confocal z-projections, dorsal views, anterior leftwards. Scale bar: 50 μm. |