FIGURE

Fig. 1

ID

ZDB-FIG-110121-7

Publication

Choudhry et al., 2011 - Alcama mediates Edn1 signaling during zebrafish cartilage morphogenesis

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Fig. 1

alcama morphants have defects in facial skeletal patterning. (A) Graphic representation of alcama RNA illustrating the position of TB and SB MOs. (B) Nomarski image of a 48 hpf WT larva with the box indicating the region shown in (C)–(E). 48 hpf control (C), alcama TB (D), and alcama SB (E) morphants stained with Zn-5 antibody. Both MOs effectively knock down alcama expression in the heart (arrowheads) and pouches (arrows). (F, G) Lateral views of 5 dpf larvae injected with control and alcama TB MOs. alcama morphants have a protruding jaw (arrows), cardiac edema (arrowheads) and an absent swim bladder (open arrows). Lateral views of Alcian blue-stained control (H) and alcama (I) morphants at 5 dpf. The line indicates the length of the pharyngeal cartilage, which is shortened in alcama morphants. The arrow points to Meckels cartilage, which is bent ventrally in alcama morphants. Scale bars: 50 μm.

Expression Data

Gene:	alcama
Antibody:	zn-5
Fish:	WT WT + MO4-alcama
Knockdown Reagent:	MO4-alcama
Anatomical Terms:	heart pharyngeal arch
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO4-alcama
Knockdown Reagent:	MO4-alcama
Observed In:	Meckel's cartilage pericardium splanchnocranium swim bladder ventral mandibular arch
Stage:	Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 349(2), Choudhry, P., Joshi, D., Funke, B., and Trede, N., Alcama mediates Edn1 signaling during zebrafish cartilage morphogenesis, 483-493, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.