FIGURE

Fig. S3

ID

ZDB-FIG-101102-9

Publication

Bagnat et al., 2010 - Cse1l Is a Negative Regulator of CFTR-Dependent Fluid Secretion

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Fig. S3

Characterization of Antibodies against Zebrafish CFTR (Related to Figures 2 and 3)

(A) In western blots of 120 hpf extracts the antibodies recognized a ~190KDa band. The intensity of the CFTR band was greatly reduced upon anti-sense morpholino knockdown.

(Bi) Whole mount staining of 120 hpf larvae showed a tight localization of CFTR to the apical membrane of gut enterocytes (arrow).

(Bii) In cross sections of the gut virtually all the CFTR protein was localized to the apical membrane, including the microvilli (arrow).

Expression Data

Gene:	cftr
Antibody:	Ab1-cftr
Fish:	WT WT + MO3-cftr
Knockdown Reagent:	MO3-cftr
Anatomical Terms:	enterocyte apical plasma membrane enterocyte microvillus whole organism
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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