FIGURE

Fig. 4

ID

ZDB-FIG-100913-7

Publication

May-Simera et al., 2010 - Bbs8, together with the planar cell polarity protein Vangl2, is required to establish left-right asymmetry in zebrafish

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Fig. 4

BBS8 and Vangl2 are required for normal Kupffer′s vesicle structure. A) Percentage spaw expression (at 15-16-somite) after bbs8-MO injection targeted to the whole embryo (WE) or the Kupffer′s vesicle (KV). Expression was randomised under both conditions. B-E) Visualisation of cilia (Ac-Tub) in the Kupffer′s vesicle in 8-somite embryos in bbs8-MO injected tri embryos. The number and/or length of the cilia are altered in the experimental conditions. F-G) Table depicting average cilia numbers and cilia length (μm) in injected and uninjected tri embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vangl2^m209/m209 TL + MO3-ttc8 vangl2^m209/m209 + MO3-ttc8
Knockdown Reagent:	MO3-ttc8
Observed In:	cilium assembly Kupffer's vesicle motile cilium Kupffer's vesicle development
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 345(2), May-Simera, H.L., Kai, M., Hernandez, V., Osborn, D.P., Tada, M., and Beales, P.L., Bbs8, together with the planar cell polarity protein Vangl2, is required to establish left-right asymmetry in zebrafish, 215-225, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.