FIGURE

Fig. S1

ID

ZDB-FIG-100809-30

Publication

Xie et al., 2010 - A novel transgenic zebrafish model for blood-brain and blood-retinal barrier development

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Fig. S1

Expression of ZO-1 in the developing BRB and BBB of zebrafish. Tg(flk1:EGFP) embryos at 2 dpf to 3 dpf were stained with rabbit anti-ZO-1; confocal images were analyzed for ZO-1 expression (red) (A-G; Alexa Fluor 568) and blood vessels (green) (A′-E′; EGFP). A&A′, lateral views; the other panels, dorsal views. (A&A′) The ZO-1 signal is high in the gut (shaded arrow) and low in the intersegmental vessels (arrows). (B&B′) At 2 dpf, ZO-1 is localized to the HV (arrows) and the HA (arrowheads). (C&C′) At 3 dpf, the HV (arrows), inner plexiform layer (shaded arrowhead) as well as the outer limiting membrane (shaded arrow) show a strong signal of ZO-1. (D&D′, E&E′) At 2 dpf, most brain vessels express ZO-1, including the BCA (arrowheads). Similar to the claudin-5 antibody, the ZO-1 antibody binds to many non-endothelial structures in the brain (shaded arrows and shaded arrowhead) besides the brain vasculature (arrows). (F&G) At 2 dpf, the ZO-1 antibody can stain the polygonal RPE cells (arrows) clearly. The insert in G is an enlarged view of the dashed square. Scale bars: 50 μm.

Expression Data

Gene:	EGFP
Antibody:	Ab2-tjp1
Fish:	s843Tg
Anatomical Terms:	basal communicating artery blood vessel endothelial cell brain brain vasculature hyaloid artery hyaloid vein outer limiting membrane retinal inner plexiform layer retinal pigmented epithelium pigmented epithelial cell (all 9)
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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