FIGURE

Fig. 5

ID

ZDB-FIG-090904-68

Publication

Arrington et al., 2009 - Extra-embryonic syndecan 2 regulates organ primordia migration and fibrillogenesis throughout the zebrafish embryo

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Fig. 5

Extra-embryonic Sdc2 controls fibronectin polymerization. (A,B) Lateral and dorsal views of wild-type (A) and yolk^sdc2MO (B) embryos at the 12-somite stage. fibronectin 1 mRNA expression patterns were normal in yolk^sdc2MO embryos. (C) Western blot experiment showing normal accumulation of fibronectin (FN) protein monomers in wild-type and yolk^sdc2MO embryos. yolk^mtx1MO embryos have decreased fibronectin monomer accumulation due to decreased transcription of fibronectin 1 (Sakaguchi et al., 2006). α-tubulin (α Tub) was used as a loading control. (D-G) Lateral and dorsal views of wild-type (D,F) and yolk^sdc2MO (E,G) embryos at the 12-somite stage reveal similar expression patterns for mRNA encoding α5 integrin (D,E) and β1 integrin (F,G).

Expression Data

Genes:	fn1a itga5 itgb1b
Fish:	AB AB + MO4-sdc2
Knockdown Reagent:	MO4-sdc2
Anatomical Term:	unspecified
Stage:	10-13 somites

Expression Detail

Gene	Fish	Conditions	Stage	Anatomy	Assay
fn1a	AB	standard conditions	10-13 somites	unspecified	ISH
fn1a	AB + MO4-sdc2	standard conditions	10-13 somites	unspecified	ISH
itga5	AB	standard conditions	10-13 somites	unspecified	ISH
itga5	AB + MO4-sdc2	standard conditions	10-13 somites	unspecified	ISH
itgb1b	AB	standard conditions	10-13 somites	unspecified	ISH
itgb1b	AB + MO4-sdc2	standard conditions	10-13 somites	unspecified	ISH

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Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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