FIGURE

Fig. 1

ID

ZDB-FIG-090223-27

Publication

Jin et al., 2009 - Definitive hematopoietic stem/progenitor cells manifest distinct differentiation output in the zebrafish VDA and PBI

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Fig. 1

Definitive erythroid cells arise in the PBI but not the VDA. (A-K) Whole-mount in situ hybridization to detect βe1-globin expression in wild-type embryos (A-E) at 22 hpf (A), 2 dpf (B), 3 dpf (C), 4 dpf (D) and 5 dpf (E); runx1^w84x mutant (F,G) and morphant (H) embryos at 22 hpf (F) and 4 dpf (G,H); and vlt^m651 embryos (I-K) at 22 hpf (I), 2 dpf (J) and 4 dpf (K). The blue arrow in E indicates βe1-globin expression in pronephros. The insets are higher magnification (20x) views of the corresponding boxed regions (blue).

Expression Data

Gene:	hbae1.1
Fish:	WT gata1a^m651/m651 runx1^hg1/hg1 WT + MO2-runx1 + MO3-runx1
Knockdown Reagents:	MO2-runx1 MO3-runx1
Anatomical Terms:	blood island erythroid progenitor cell post-vent region erythroid lineage cell post-vent region erythroid progenitor cell pronephros
Stage Range:	26+ somites to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	runx1^hg1/hg1 WT + MO2-runx1 + MO3-runx1
Knockdown Reagents:	MO2-runx1 MO3-runx1
Observed In:	blood island hematopoietic multipotent progenitor cell definitive hemopoiesis erythroid progenitor cell ventral wall of dorsal aorta hematopoietic multipotent progenitor cell
Stage:	Day 4

Phenotype Detail

Acknowledgments

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