CRISPR

CRISPR2-tmem88b,si:dkey-85k7.8

ID

ZDB-CRISPR-230807-5

Previous Names

CRISPR2-tmem88b

Targets

si:dkey-85k7.8 (1)
tmem88b (1)

Sequence

5' - GCTAATCCTCACTCCTGCTATCGTCA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
szy208	tmem88b
szy210	tmem88b

Expression

Gene expression in Wild Types + CRISPR2-tmem88b,si:dkey-85k7.8

No data available

Phenotype

Phenotype resulting from CRISPR2-tmem88b,si:dkey-85k7.8

No data available

Phenotype of all Fish created by or utilizing CRISPR2-tmem88b,si:dkey-85k7.8

Phenotype	Fish	Conditions	Figures
pharyngeal pouch endodermal cell fate specification process quality, ameliorated	tmem88b^{szy208/szy208}; tmem88a^{szy207/szy207}	chemical treatment by environment: N-[(4-chlorophenyl)-(2-methyl-1H-indol-3-yl)methyl]-2-pyridinamine	Figure 5. from Liu et al., 2023
pharyngeal pouch nkx2.3 expression amount, ameliorated	tmem88b^{szy208/szy208}; tmem88a^{szy207/szy207}	chemical treatment by environment: N-[(4-chlorophenyl)-(2-methyl-1H-indol-3-yl)methyl]-2-pyridinamine	Figure 5. from Liu et al., 2023
pharyngeal pouch poorly differentiated, abnormal	tmem88b^{szy208/szy208}; tmem88a^{szy207/szy207}	standard conditions	Figure 3. from Liu et al., 2023
pharyngeal pouch nkx2.3 expression decreased amount, abnormal	tmem88b^{szy208/szy208}; tmem88a^{szy207/szy207}	control	Figure 3. , Figure 5. from Liu et al., 2023
pharyngeal pouch endodermal cell fate specification decreased process quality, abnormal	tmem88b^{szy208/szy208}; tmem88a^{szy207/szy207}	control	Figure 5. from Liu et al., 2023
pharyngeal endoderm ab14-ctnnb labeling increased amount, abnormal	tmem88b^{szy208/szy208}; tmem88a^{szy207/szy207}; ioz15Tg	standard conditions	Figure 5. from Liu et al., 2023
pharyngeal pouch poorly differentiated, abnormal	tmem88b^{szy208/szy208}; tmem88a^{szy207/szy207}; ioz15Tg	standard conditions	Figure 3. from Liu et al., 2023
pharyngeal pouch canonical Wnt signaling pathway increased process quality, abnormal	tmem88b^{szy208/szy208}; tmem88a^{szy207/szy207}; ioz15Tg	standard conditions	Figure 5. from Liu et al., 2023
pharyngeal pouch mCherry expression decreased amount, abnormal	tmem88b^{szy208/szy208}; tmem88a^{szy207/szy207}; ioz15Tg	standard conditions	Figure 3. from Liu et al., 2023

Citations