FIGURE SUMMARY
Title

Molecular cloning and expression of the col2a1a and col2a1b genes in the medaka, Oryziaslatipes

Authors
Matsumoto, T., Deguchi, T., Kawasaki, T., Yuba, S., and Sato, J.
Source
Full text @ Gene Expr. Patterns
Fig. 2

Expression patterns of col2a1a and col2a1b mRNAs in medaka embryo detected by whole-mount insitu hybridization at 1–2 dpf using the col2a1a probe (A, B, E, F, I, and J) and col2a1b probe (C, D, G, H, K, and L). Results at 1 dpf (A–D) and 2 dpf (E–L) in the dorsal view (A, C, E, and G) and lateral view (B, D, F, and H). (I–L) is a transverse section of the specimen at 2 dpf. (I and K) is the anterior notochord, (J and L) is the posterior notochord. The broken line of (I–L) is an outline of the neural tube and notochord. The sense probe showed no signals in any tissues through all of the stages tested (data not shown). axm, axial mesoderm; fp, floor plate; hy, hypochord; nt, notochord; ot, otic vesicle. Scale bar = 250 μm (A–H), 20 μm (I–L).
Fig. 3

Expression patterns of col2a1a and col2a1b mRNA in medaka embryo detected by whole-mount insitu hybridization, using the col2a1a probe (A–D) and col2a1b probe (E–H) at 3–4 dpf. Results at 3 dpf (A and E) and 4 dpf (B–D and F–H), in the dorsal view (A, B, E, and F), ventral view (C and G) and lateral view (D and H). The sense probe showed no signals in any tissues through all the stages tested (data not shown). Arrow head is a tip of notochord. bh, basihyal; ch, ceratohyal; ed, endoskeletal disc; ff, fin fold; mf, membranous fin; ot, otic vesicle; pq, palatoquadrate; sc, scapulocoracoid; su, suture. Scale bar = 200 μm.
Fig. 4

Expression patterns of col2a1a and col2a1b mRNA in medaka embryo detected by whole-mount insitu hybridization, using the col2a1a probe (A–D and I–L), col2a1b probe (E–H and M–P) at 5, 6, 8 dpf. Results at 5 dpf (A–I and M), 6 dpf (J and N) and 8 dpf (K, L, O, and P) in the dorsal view (A and E), ventral view (B, F, J, K, N, and O) and frontal view (C and G). (I and M) is a transverse section of the head. (L) is a right pectoral fin of the specimen of (K). (P) is a right pectoral fin of the specimen of (O). Arrow heads are hypural bones. bb, basibranchial; bh, basihyal; ch, ceratohyal; ed, endoskeletal disc; ep, ethmoid plate; ff, fin fold; m, Meckel’s cartilage; mf, membranous fin; ot, otic vesicle; pq, palatoquadrate; sc, scapulocoracoid; su, suture. Scale bar = 200 μm (A–H, J, K, N, and O), 50 μm (I and M).
Acknowledgments
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Reprinted from Gene expression patterns : GEP, 12(1-2), Matsumoto, T., Deguchi, T., Kawasaki, T., Yuba, S., and Sato, J., Molecular cloning and expression of the col2a1a and col2a1b genes in the medaka, Oryziaslatipes, 46-52, Copyright (2012) with permission from Elsevier. Full text @ Gene Expr. Patterns
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