Transgenic Construct
Tg(atp1a1a.4:EGFP)
- ID
- ZDB-TGCONSTRCT-070723-1
- Name
- Tg(atp1a1a.4:EGFP)
- Previous Names
-
- Na,K-ATPase alpha1A4:GFP (1)
- Type
- engineered_plasmid
- Regulatory Regions
- Coding Sequences
- [Em λ][Ex λ]
- Fpbase:EGFP
- Contains Sequences
- None
- Note
-
Liu et al. 2007, ZDB-PUB-070212-38 : The Na,K-ATPase alpha1A4:GFP transgenic line was created by amplifying an 8112 base-pair (bp) fragment of the Na,K- ATPase alpha1A4-subunit promoter (cDNA accession: NM_131689) from genomic DNA using nested PCR with the following primers: LNaK-F1: 5-GGTCGCTGGGCTAAGTCACAGAC-3 LNaK-F2: 5-TGGGCTAAGTCACAGACCGCCTA-3 LNaK-R: 5-TCCACTCACTCCAAGCCCCATTT-3 NaKB-R2: 5-CGCGGATCCCTCACTCCAAGCCCCATTTTCCT-3. The 8112 bp fragment was cloned in pGI upstream of EGFP and injected as a circular plasmid into TuAB wild-type embryos at the 1-cell stage. Founders were screened by GFP fluorescence in the pronephros and were out crossed to create a stable transgenic line.
Plasmid Map
None
Genomic Features
That Utilize Tg(atp1a1a.4:EGFP) Construct
| Genomic Feature | Affected Genomic Regions |
|---|---|
| zf34Tg |
1 - 1 of 1
Transgenics
That Utilize Tg(atp1a1a.4:EGFP) Construct
| Fish | Affected Genomic Regions | Phenotype | Gene Expression |
|---|---|---|---|
| vhlhu2117/hu2117; zf34Tg |
2 figures
from
Noonan et al., 2016
|
2 figures
from
Noonan et al., 2016
|
|
| zf34Tg |
|
9 figures
from
6 publications
|
|
| zf34Tg + CRISPR1-crkl |
|
Fig. 2 from Lopez-Rivera et al., 2017 | |
| zf34Tg + CRISPR1-ret |
|
Fig. 2 from Lopez-Rivera et al., 2017 | |
| zf34Tg + CRISPR1-snap29 |
|
Fig. 2 from Lopez-Rivera et al., 2017 |
1 - 5 of 22 Show all
Sequence Information
Other Construct Pages
None
Citations