PUBLICATION

Spadetail-dependent cell compaction of the dorsal zebrafish blastula

Authors
Warga, R.M. and Nüsslein-Volhard, C.
ID
ZDB-PUB-981208-31
Date
1998
Source
Developmental Biology   203: 116-121 (Journal)
Registered Authors
Nüsslein-Volhard, Christiane, Warga, Rachel M.
Keywords
organizer; mesoderm; endoderm; axial-paraxial boundary; fate map; morphogenesis; regionalization; single cell labeling; spadetail
MeSH Terms
  • Clone Cells/metabolism
  • Immunohistochemistry
  • Animals
  • T-Box Domain Proteins
  • Embryonic Development
  • Zebrafish/embryology*
  • Mesoderm/metabolism
  • Mutation/genetics
  • Transcription Factors/genetics*
  • Blastocyst/physiology*
  • Gene Expression Regulation, Developmental/genetics*
  • Zebrafish Proteins*
  • Cell Differentiation/genetics
  • Notochord/growth & development*
(all 14)
PubMed
9806777 Full text @ Dev. Biol.
Abstract
The dorsal marginal zone of the zebrafish blastula, equivalent to the amphibian Spemann organizer, is destined to become the tissues of the notochord and prechordal plate. Preceding gastrulation in the zebrafish, we find that these future mesendodermal cells acquire a cohesive cell behavior characterized by flattening and maximization of intercellular contacts, somewhat resembling cell compaction in mouse blastocysts. This behavior may suppress cell intermingling. Surprisingly, this blastula cell compaction requires normal function of spadetail, a gene known to be necessary for the dorsal convergent cell movement of paraxial mesoderm later in the gastrula. We propose that spadetail-dependent cell compaction subtly controls the early mixing and dispersal of dorsal cells that coalesce into the prospective organizer region. This early process may be necessary for the correct location of the boundary separating axial and paraxial cells.
Genes / Markers
Figures
Figure Gallery (3 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b104
    		Indel
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    No data available
    Fish
    Fish
    tbx16b104/b104 (AB)
    1 - 1 of 1
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    Antibodies
    Name Type Antigen Genes Isotypes Host Organism
    Ab1-tapolyclonalRabbit
    1 - 1 of 1
    Show
    Orthology
    No data available
    Engineered Foreign Genes
    No data available
    Mapping
    No data available
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    Citation
    Warga, R.M. and Nüsslein-Volhard, C. (1998) Spadetail-dependent cell compaction of the dorsal zebrafish blastula. Developmental Biology. 203:116-121.