PUBLICATION
Cloning and sequencing of cDNAs encoding ribonucleotide reductase from zebrafish Danio rerio
- Authors
- Mathews, C.Z., Sjoberg, B.M., and Karlsson, M.
- ID
- ZDB-PUB-970324-10
- Date
- 1996
- Source
- Molecular marine biology and biotechnology 5(4): 284-287 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Ribonucleotide Reductases/genetics*
- Sequence Homology, Amino Acid
- Sequence Analysis, DNA
- Cloning, Molecular
- Zebrafish/genetics*
- Molecular Sequence Data
- Introns
- Species Specificity
- Animals
- Base Sequence
- PubMed
- 8983196
Citation
Mathews, C.Z., Sjoberg, B.M., and Karlsson, M. (1996) Cloning and sequencing of cDNAs encoding ribonucleotide reductase from zebrafish Danio rerio. Molecular marine biology and biotechnology. 5(4):284-287.
Abstract
We have cloned and sequenced cDNAs coding for the R1 and R2 proteins of ribonucleotide reductase from zebrafish (Danio rerio). This ribonucleotide reductase shows high amino acid sequence identity to those of other vertebrates. The R1 cDNA has a coding sequence of 2382 bp, yielding a 794 amino acid protein, and the R2 cDNA has a coding sequence of 1158 bp, yielding a 386 amino acid protein. The zebrafish R1 shows 94% similarity and R2 shows 91% similarity to the human R1 and R2, respectively. The similarity extends to intron positions, of which the equivalent of mouse R2 intron 3 has been studied.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping