PUBLICATION

Mutations disrupting the ordering and topographic mapping of axons in the retinotectal projection of the zebrafish, Danio rerio

Authors
Trowe, T., Klostermann, S., Baier, H., Granato, M., Crawford, A.D., Grunewald, B., Hoffman, H., Karlstrom, R.O., Meyer, S.U., Muller, B., Richter, S., Nüsslein-Volhard, C., and Bonhoeffer, F.
ID
ZDB-PUB-970210-36
Date
1996
Source
Development (Cambridge, England)   123: 439-450 (Journal)
Registered Authors
Baier, Herwig, Bonhoeffer, Friedrich, Crawford, Alexander, Granato, Michael, Grunewald, Barbara, Hoffman, Heinke, Karlstrom, Rolf, Nüsslein-Volhard, Christiane, Trowe, Torsten
Keywords
retinotectal projection; topographic mapping; zebrafish; Danio rerio; visual system
MeSH Terms
  • Gene Expression Regulation, Developmental
  • Superior Colliculi/embryology*
  • Animals
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish/physiology
  • Axons/physiology*
  • Phenotype
  • Mutation*
  • Retina/embryology*
  • Visual Pathways/embryology*
  • Brain Mapping
(all 12)
PubMed
9007261 Full text @ Development
Abstract
Retinal ganglion cells connect to their target organ, the rectum, in a highly ordered fashion. We performed a large-scale screen for mutations affecting the retinotectal projection of the zebrafish, which resulted in the identification of 114 mutations. 44 of these mutations disturb either the order of RGC axons in the optic nerve and tract, the establishment of a topographic map on the tectum, or the formation of proper termination fields. Mutations in three genes, boxer, dackel and pinscher, disrupt the sorting of axons in the optic tract but do not affect mapping on the tectum. In these mutants, axons from the dorsal retina grow along both the ventral and the dorsal branch of the optic tract. Mutations in two genes, nevermind and who-cares, affect the dorsoventral patterning of the projection. In embryos homozygous for either of these mutations, axons from dorsal retinal ganglion cells terminate ventrally and dorsally in the tectum. In nevermind, the retinotopic order of axons along the optic nerve and tract is changed in a characteristic way as well, while it appears to be unaffected in who-cares. Two mutations in two complementation groups, gnarled and macho, affect the anteroposterior patterning of the projection. In these mutants, nasodorsal axons branch and terminate too soon in the anterior tectum. In 27 mutants belonging to six complementation groups, retinal axons do not form normal termination fields. Some implications for models concerning the formation of topographic projections are discussed.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
No data available
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ta229f
    Point Mutation
    tb21
      Point Mutation
      tb241a
        Unknown
        tc37
          Unknown
          tc38
            Unknown
            tc236z
              Unknown
              tc257z
                Point Mutation
                tc265y
                  Unknown
                  tc265z
                    Unknown
                    tc281
                      Unknown
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      No data available
                      Fish
                      Antibodies
                      No data available
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      No data available
                      Mapping
                      No data available