PUBLICATION

Genetic analysis of fin formation in the zebrafish, Danio rerio

Authors

van Eeden, F.J., Granato, M., Schach, U., Brand, M., Furutani-Seiki, M., Haffter, P., Hammerschmidt, M., Heisenberg, C.P., Jiang, Y.J., Kane, D.A., Kelsh, R.N., Mullins, M.C., Odenthal, J., Warga, R.M., and Nüsslein-Volhard, C.

ID

ZDB-PUB-970210-21

Date

1996

Source

Development (Cambridge, England) 123: 255-262 (Journal)

Registered Authors

Brand, Michael, Furutani-Seiki, Makoto, Granato, Michael, Haffter, Pascal, Hammerschmidt, Matthias, Heisenberg, Carl-Philipp, Jiang, Yun-Jin, Kane, Donald A., Kelsh, Robert, Mullins, Mary C., Nüsslein-Volhard, Christiane, Odenthal, Joerg, van Eeden, Freek, Warga, Rachel M.

Keywords

embryonic fin fold; pectoral fins; sonic hedgehog; adult fins; skin; zebrafish

MeSH Terms

Larva/genetics
Larva/growth & development
Tail/embryology
Skin/embryology
Extremities/embryology*
Extremities/growth & development
Zebrafish/embryology*
Zebrafish/genetics*
Zebrafish/growth & development
Animals
Phenotype
Mutation*
Pectoralis Muscles

(all 13)

PubMed

9007245 Full text @ Development

Abstract

In the zebrafish, Danio rerio, a caudal and pectoral fin fold develop during embryogenesis. At larval stages the caudal fin fold is replaced by four different fins, the unpaired anal, dorsal and tail fins. In addition the paired pelvic fins are formed. We have identified a total of 118 mutations affecting larval fin formation. Mutations in 11 genes lead to abnormal morphology or degeneration of both caudal and pectoral fin folds. Most mutants survive to adulthood and form a surprisingly normal complement of adult fins. Mutations in nine genes result in an increased or reduced size of the pectoral fins. Interestingly, in mutants of one of these genes, dackel (dak), pectoral fin buds form initially, but later the fin epithelium fails to expand. Expression of sonic hedgehog mRNA in the posterior mesenchyme of the pectoral fin bud is initiated in dak embryos, but not maintained. Mutations in five other genes affect adult fin but not larval fin development. Two mutants, longfin (lof) and another longfin (alf) have generally longer fins. Stein und bein (sub) has reduced dorsal and pelvic fins, whereas finless (fls) and wanda (wan) mutants affect all adult fins. Finally, mutations in four genes causing defects in embryonic skin formation will be briefly reported.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
shha	ext2^tw25e/tw25e	standard conditions	Prim-5		pectoral fin bud	ISH	Fig. 5
shha	ext2^tw25e/tw25e	standard conditions	Prim-15	Not Detected	pectoral fin bud	ISH	Fig. 5
shha	WT	standard conditions	Prim-5		pectoral fin bud	ISH	Fig. 5
shha	WT	standard conditions	Prim-15		pectoral fin bud	ISH	Fig. 5

1 - 4 of 4

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
acvr1l^{tm110b/tm110b}	standard conditions	Long-pec	caudal fin ventral region hypoplastic, abnormal	Table 1
acvr1l^{tm110b/tm110b}	standard conditions	Long-pec	median fin fold hypoplastic, abnormal	Table 2
acvr1l^{tm110b/tm110b}	standard conditions	Long-pec	whole organism wholly dorsalized, abnormal	Table 1
bmp1a^tf5/+; bmp1a^tm317a/+	standard conditions	Long-pec	ventral fin fold undulate, abnormal	Fig. 2
bmp1a^tf5/tf5	standard conditions	26+ somites to Day 5	median fin fold decreased length, abnormal	text only
bmp1a^tf5/tf5	standard conditions	Long-pec	anal fin lepidotrichium decreased size, abnormal	Table 2
bmp1a^tf5/tf5	standard conditions	Long-pec	caudal fin lepidotrichium decreased size, abnormal	Table 2
bmp1a^tf5/tf5	standard conditions	Long-pec	dorsal fin lepidotrichium decreased size, abnormal	Table 2
bmp1a^tf5/tf5	standard conditions	Long-pec	fin undulate, abnormal	Table 1
bmp1a^tf5/tf5	standard conditions	Long-pec	median fin fold undulate, abnormal	Table 2

1 - 10 of 1,037

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Mutations / Transgenics

Allele	Type	Affected Genomic Region
dt2	Unknown	lof
dti216c	Point Mutation	smad5
dty40	Point Mutation	smad5
ta50	Unknown	ddf
ta52b	Point Mutation	mib1
ta72a	Point Mutation	bmp2b
ta84	Unknown	hmcn1
ta90	Point Mutation	frem2a
ta206	Unknown	smad5
tb22	Unknown	hmcn1

1 - 10 of 144

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Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
acvr1l^{tm110b/tm110b}
acvr1l^{tm110b/tm110b} (TU)
bmp1a^tf5/+; bmp1a^tm317a/+
bmp1a^tf5/tf5
bmp1a^th42/th42
bmp1a^{tm317a/tm317a}
bmp1a^tp34/tp34
bmp1a^tp207/tp207
bmp1a^ty69/ty69
bmp1a^tz252/tz252

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Antibodies

Orthology

Engineered Foreign Genes

Mapping

van Eeden et al., 1996 ZDB-PUB-970210-21 PMID:9007245

Genetic analysis of fin formation in the zebrafish, Danio rerio

van Eeden et al., 1996

ZDB-PUB-970210-21
PMID:9007245