PUBLICATION

Neural degeneration mutants in the zebrafish, Danio rerio

Authors

Furutani-Seiki, M., Jiang, Y.J., Brand, M., Heisenberg, C.P., Houart, C., Beuchle, D., van Eeden, F.J.M., Granato, M., Haffter, P., Hammerschmidt, M., Kane, D.A., Kelsh, R.N., Mullins, M.C., Odenthal, J., and Nüsslein-Volhard, C.

ID

ZDB-PUB-970210-19

Date

1996

Source

Development (Cambridge, England) 123: 229-239 (Journal)

Registered Authors

Brand, Michael, Furutani-Seiki, Makoto, Granato, Michael, Haffter, Pascal, Hammerschmidt, Matthias, Heisenberg, Carl-Philipp, Houart, Corinne, Jiang, Yun-Jin, Kane, Donald A., Kelsh, Robert, Mullins, Mary C., Nüsslein-Volhard, Christiane, Odenthal, Joerg, van Eeden, Freek

Keywords

neural degeneration; apoptosis; zebrafish; neural plate patterning; nervous system development; tectum; retina

MeSH Terms

Zebrafish/embryology*
Zebrafish/genetics*
Body Patterning/genetics
Mutation*
Nerve Degeneration/genetics*
Genetic Markers
Phenotype
Apoptosis/genetics
In Situ Hybridization
Animals

(all 10)

PubMed

9007243 Full text @ Development

Abstract

Forty zebrafish mutants with localized or general neural degeneration are described. The onset and duration of degeneration and the distribution of ectopically dying cells are specific characteristics of each mutant. Mutants are classified into four groups by these parameters. Class I: late focal neural degeneration mutants. These 18 mutants have restricted cell death mainly in the tectum and the dorsal hindbrain after 36 hours. The degeneration does not spread and disappears at later stages of development. Class II: early focal neural degeneration mutants. Ten mutants in this class exhibit transient restricted degeneration affecting mainly the diencephalon, the hindbrain and the spinal cord at 20 hours. The midbrain is less affected. The degeneration shifts to the dorsal diencephalon and the tectum at 36 hours. Class III: late spreading neural degeneration mutants. The 8 mutants in this class display a degeneration that is first seen in the tectum and subsequently spreads throughout the nervous system from 36 hours on. Class IV: early general neural degeneration mutants. This class of four mutants already shows overall cell degeneration in the nervous system at the 15-somite stage. Three of the class I mutants show a change in the pattern of gene expression in the anlage of a brain structure prior to the onset of degeneration. These results suggest that focal cell death may be a useful clue for the detection of early patterning defects of the vertebrate nervous system in regions devoid of visible landmarks.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
ascl1a	aoi^{tr222d/tr222d}	standard conditions	10-13 somites	basal plate midbrain region	ISH	Fig. 2
ascl1a	aoi^{tr222d/tr222d}	standard conditions	10-13 somites	forebrain dorsal region	ISH	Fig. 2
ascl1a	aoi^{tr222d/tr222d}	standard conditions	10-13 somites	forebrain ventral region	ISH	Fig. 2
ascl1a	aoi^{tr222d/tr222d}	standard conditions	10-13 somites	midbrain ventral region	ISH	Fig. 2
ascl1a	aoi^{tr222d/tr222d}	standard conditions	10-13 somites	midbrain hindbrain boundary neural rod	ISH	Fig. 2
ascl1a	aoi^{tr222d/tr222d}	standard conditions	10-13 somites	rhombomere 2	ISH	Fig. 2
ascl1a	unm_tc294a^{tc294a/tc294a}	standard conditions	10-13 somites	basal plate midbrain region	ISH	Fig. 2
ascl1a	unm_tc294a^{tc294a/tc294a}	standard conditions	10-13 somites	forebrain dorsal region	ISH	Fig. 2
ascl1a	unm_tc294a^{tc294a/tc294a}	standard conditions	10-13 somites	forebrain ventral region	ISH	Fig. 2
ascl1a	unm_tc294a^{tc294a/tc294a}	standard conditions	10-13 somites	midbrain ventral region	ISH	Fig. 2

1 - 10 of 22

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
aoi^{tr222d/tr222d}	standard conditions	Prim-25	apoptotic process increased occurrence, abnormal	Fig. 2
aoi^{tr222d/tr222d}	standard conditions	Prim-25	brain degenerate, abnormal	Fig. 2
aoi^{tr222d/tr222d}	standard conditions	Prim-25	cerebellum apoptotic, abnormal	Fig. 2
aoi^{tr222d/tr222d}	standard conditions	Prim-25	eye decreased size, abnormal	Table 1
aoi^{tr222d/tr222d}	standard conditions	Prim-25	head deformed, abnormal	Table 1
aoi^{tr222d/tr222d}	standard conditions	Prim-25	hindbrain dorsal region apoptotic, abnormal	Fig. 2
aoi^{tr222d/tr222d}	standard conditions	Prim-25	neuroepithelial cell apoptotic, abnormal	Fig. 2
aoi^{tr222d/tr222d}	standard conditions	Prim-25	optic tectum apoptotic, abnormal	Fig. 2
aoi^{tr222d/tr222d}	standard conditions	Prim-25	retinal neural layer apoptotic, abnormal	Fig. 2
awa^tm17/tm17	standard conditions	14-19 somites	central nervous system degenerate, abnormal	Table 1

1 - 10 of 346

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Mutations / Transgenics

Allele	Type	Affected Genomic Region
ta53b	Unknown	unm_ta53b
ta53c	Point Mutation	pold1
tc1	Unknown	yug
tc11a	Unknown	unm_tc11a
tc11c	Unknown	ger
tc31	Unknown	unm_tc31
tc233b	Unknown	unm_tc233b
tc234e	Unknown	unm_tc234e
tc265z	Unknown	brd
tc294a	Unknown	unm_tc294a

1 - 10 of 72

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Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
aoi^{tr222d/tr222d}
awa^tm17/tm17
bch^{ti282b/ti282b}
blc^{th204b/th204b}
blc^ts23/ts23
blc^ty89/ty89
brd^{tc265z/tc265z}
brd^tl21/tl21
brd^tl41/tl41
brd^tm42z/tm42z

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Furutani-Seiki et al., 1996 ZDB-PUB-970210-19 PMID:9007243

Neural degeneration mutants in the zebrafish, Danio rerio

Furutani-Seiki et al., 1996

ZDB-PUB-970210-19
PMID:9007243