PUBLICATION

Mutations affecting neurogenesis and brain morphology in the zebrafish, Danio rerio

Authors
Jiang, Y.J., Brand, M., Heisenberg, C.P., Beuchle, D., Furutani-Seiki, M., Kelsh, R.N., Warga, R.M., Granato, M., Haffter, P., Hammerschmidt, M., Kane, D.A., Mullins, M.C., Odenthal, J., van Eeden, F.J., and Nüsslein-Volhard, C.
ID
ZDB-PUB-970210-17
Date
1996
Source
Development (Cambridge, England)   123: 205-216 (Journal)
Registered Authors
Brand, Michael, Furutani-Seiki, Makoto, Granato, Michael, Haffter, Pascal, Hammerschmidt, Matthias, Heisenberg, Carl-Philipp, Jiang, Yun-Jin, Kane, Donald A., Kelsh, Robert, Mullins, Mary C., Nüsslein-Volhard, Christiane, Odenthal, Joerg, van Eeden, Freek, Warga, Rachel M.
Keywords
neurogenesis; neurogenic genes; somitogenesis; hindbrain; brain ventricles; adhesion; primary neurons; zebrafish
MeSH Terms
  • Neuroglia/cytology
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Cerebral Ventricles/embryology
  • Phenotype
  • Cell Differentiation/genetics
  • Animals
  • Neural Crest/cytology
  • Neural Crest/embryology
  • Neurons/cytology
  • Muscle, Skeletal/embryology
  • Brain/embryology*
  • Brain/pathology
  • Hyperplasia
  • Mutagenesis*
  • Somites/physiology
(all 16)
PubMed
9007241 Full text @ Development
Abstract
In a screen for embryonic mutants in the zebrafish a large number of mutants were isolated with abnormal brain morphology. We describe here 26 mutants in 13 complementation groups that show abnormal development of large regions of the brain. Early neurogenesis is affected in white tail (wit). During segmentation stages, homozygous wit embryos display an irregularly formed neural keel, particularly in the hindbrain. Using a variety of molecular markers, a severe increase in the number of various early differentiating neurons can be demonstrated. In contrast, late differentiating neurons, radial glial cells and some nonneural cell types, such as the neural crest-derived melanoblasts, are much reduced. Somitogenesis appears delayed. In addition, very reduced numbers of melanophores are present posterior to the mid-trunk. The wit phenotype is reminiscent of neurogenic mutants in Drosophila, such as Notch or Delta. In mutant parachute (pac) embryos the general organization of the hindbrain is disturbed and many rounded cells accumulate loosely in the hindbrain and midbrain ventricles. Mutants in a group of 6 genes, snakehead(snk), natter (nat), otter (ott), fullbrain (ful), viper (vip) and white snake (wis) develop collapsed brain ventricles, before showing signs of general degeneration. atlantis (atl), big head (bid), wicked brain (win), scabland (sbd) and eisspalte (ele) mutants have different malformation of the brain folds. Some of them have transient phenotypes, and mutant individuals may grow up to adults.
Genes / Markers
Figures
Figure Gallery (9 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m115
    Unknown
    m133
      Unknown
      m157
        Unknown
        m273
          Unknown
          m427
            Unknown
            m523
              Unknown
              m628
                Point Mutation
                m673
                  Point Mutation
                  ta52b
                    Point Mutation
                    ta52e
                      Unknown
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                      Human Disease / Model
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                      Sequence Targeting Reagents
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                      Fish
                      Antibodies
                      Orthology
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                      Engineered Foreign Genes
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                      Mapping
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