PUBLICATION

A genetic linkage map for the zebrafish

Authors
Postlethwait, J., Johnson, S., Midson, C.N., Talbot, W.S., Gates, M., Ballenger, E.W., Africa, D., Andrews, R., Carl, T., Eisen, J.S., Horne, S., Kimmel, C.B., Hutchinson, M., Johnson, M., and Rodriguez, A.
ID
ZDB-PUB-961014-888
Date
1994
Source
Science (New York, N.Y.)   264: 699-703 (Journal)
Registered Authors
Eisen, Judith S., Gates, Michael A., Horne-Badovinac, Sally, Johnson, Stephen L., Kimmel, Charles B., Montgomery, Rebecca, Postlethwait, John H., Talbot, William S.
Keywords
none
MeSH Terms
  • Chromosome Mapping*
  • Zebrafish/genetics*
  • Genetic Markers
  • Software
  • Female
  • Cloning, Molecular
  • Polymerase Chain Reaction
  • Animals
  • Phenotype
  • Repetitive Sequences, Nucleic Acid
  • Genotype
  • Male
  • Mutation
(all 13)
PubMed
8171321 Full text @ Science
Abstract
To facilitate molecular genetic analysis of vertebrate development, haploid genetics was used to construct a recombination map for the zebrafish Danio (Brachydanio) rerio. The map consists of 401 random amplified polymorphic DNAs (RAPDs) and 13 simple sequence repeats spaced at an average interval of 5.8 centimorgans. Strategies that exploit the advantages of haploid genetics and RAPD markers were developed that quickly mapped lethal and visible mutations and that placed cloned genes on the map. This map is useful for the position-based cloning of mutant genes, the characterization of chromosome rearrangements, and the investigation of evolution in vertebrate genomes.
Genes / Markers
Figures
No images available
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b2
    Unknown
    b4
      Insertion
      b5
        Small Deletion
        b16
          Deficiency
          b109
            Small Deletion
            b160
              Indel
              b212
                Unknown
                n1
                  Small Deletion
                  t1
                    Point Mutation
                    1 - 9 of 9
                    Show
                    Human Disease / Model
                    No data available
                    Sequence Targeting Reagents
                    No data available
                    Fish
                    Antibodies
                    Orthology
                    Engineered Foreign Genes
                    No data available
                    Mapping