PUBLICATION

Regulatory gene expression boundaries demarcate sites of neuronal differentiation in the embryonic zebrafish forebrain

Authors

Macdonald, R., Xu, Q., Barth, K.A., Mikkola, I., Holder, N., Fjose, A., Krauss, S., and Wilson, S.W.

ID

ZDB-PUB-961014-719

Date

1994

Source

Neuron 13: 1039-1053 (Journal)

Registered Authors

Barth, Anukampa, Fjose, Anders, Holder, Nigel, Krauss, Stefan, Macdonald, Rachel, Wilson, Steve, Xu, Qiling

Keywords

none

MeSH Terms

Transcription Factors/genetics
Prosencephalon/embryology*
Cell Differentiation
Zebrafish
Animals
Neurons/cytology*
PAX2 Transcription Factor
Proto-Oncogene Proteins/genetics
Repressor Proteins
Homeodomain Proteins*
Zebrafish Proteins*
DNA-Binding Proteins/genetics
Eye Proteins
Forkhead Transcription Factors
Neural Pathways/embryology
In Situ Hybridization
Receptor Protein-Tyrosine Kinases/genetics
Morphogenesis
Genes, Regulator
Gene Expression Regulation, Developmental*
Paired Box Transcription Factors
Wnt Proteins

(all 22)

PubMed

7946344 Full text @ Neuron

Abstract

During development of the zebrafish forebrain, a simple scaffold of axon pathways is pioneered by a small number of neurons. We show that boundaries of expression domains of members of the eph, forkhead, pax, and wnt gene families correlate with the positions at which these neurons differentiate and extend axons. Analysis of genetically or experimentally altered forebrains indicates that if a boundary is maintained, there is appropriate neural differentiation with respect to the boundary. Conversely, in the absence of a boundary, there is concomitant disruption of neural patterning. We also show that a strip of cells within the dorsal diencephalon shares features with ventral midline cells. This strip of cells fails to develop in mutant fish in which specification of the ventral CNS is disrupted, suggesting that its development may be regulated by the same inductive pathways that pattern the ventral midline.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
epha4a	WT	standard conditions	20-25 somites		forebrain dorsal region	ISH	Fig. 1
epha4a	WT	standard conditions	Prim-5		forebrain	ISH	Fig. 2
epha4a	WT	standard conditions	14-19 somites		forebrain	ISH	Fig. 3
epha4a	WT	standard conditions	Prim-5		forebrain	ISH	Fig. 1
epha4a	WT	standard conditions	26+ somites		forebrain	ISH	Fig. 3
epha4a	WT	standard conditions	14-19 somites		forebrain	ISH	Fig. 2
epha4a	WT	standard conditions	Prim-5		hindbrain	ISH	Fig. 1
epha4a	WT	standard conditions	20-25 somites	Not Detected	immature eye	ISH	Fig. 1
epha4a	WT	standard conditions	20-25 somites		optic stalk proximal region	ISH	Fig. 1
epha4b	WT	standard conditions	14-19 somites		diencephalon	ISH	Fig. 1

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
Df(Chr12:hhex,ndr2)b16/b16	standard conditions	Prim-5	forebrain axon guidance disrupted, abnormal	Fig. 5
Df(Chr12:hhex,ndr2)b16/b16	standard conditions	Prim-5	forebrain commissural neuron axon guidance disrupted, abnormal	Fig. 5
Df(Chr12:hhex,ndr2)b16/b16	standard conditions	Prim-15	diencephalic white matter lacks all parts of type tract of the postoptic commissure, abnormal	Fig. 5
Df(Chr12:hhex,ndr2)b16/b16	standard conditions	Prim-15	diencephalon has fewer parts of type neuron, abnormal	Fig. 5
Df(Chr12:hhex,ndr2)b16/b16	standard conditions	Prim-15	forebrain commissural neuron axon guidance disrupted, abnormal	Fig. 5
Df(Chr12:hhex,ndr2)b16/b16	standard conditions	Prim-15	nucleus of the medial longitudinal fasciculus synencephalon has fewer parts of type neuron, abnormal	Fig. 5
WT	chemical treatment: pharmaceutical	10-13 somites to 14-19 somites	axon guidance process quality, normal	Fig. 6
WT	chemical treatment: pharmaceutical	10-13 somites to 14-19 somites	diencephalon decreased size, abnormal	Fig. 6
WT	chemical treatment: pharmaceutical	10-13 somites to 14-19 somites	forebrain antero-dorsal region decreased size, abnormal	Fig. 6
WT	chemical treatment: pharmaceutical	10-13 somites to 14-19 somites	forebrain commissural neuron axon guidance process quality, normal	Fig. 6

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
b16		Deficiency	hhex ndr2

Human Disease / Model

Sequence Targeting Reagents

Fish

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Macdonald et al., 1994 ZDB-PUB-961014-719 PMID:7946344

Regulatory gene expression boundaries demarcate sites of neuronal differentiation in the embryonic zebrafish forebrain

Macdonald et al., 1994

ZDB-PUB-961014-719
PMID:7946344