PUBLICATION

Role of the floor plate in axonal patterning in the zebrafish CNS

Authors
Hatta, K.
ID
ZDB-PUB-961014-416
Date
1992
Source
Neuron   9: 629-642 (Journal)
Registered Authors
Hatta, Kohei
Keywords
none
MeSH Terms
  • Motor Neurons/cytology
  • Motor Neurons/ultrastructure
  • Neurons/cytology*
  • Neurons/ultrastructure
  • Spinal Cord/anatomy & histology*
  • Spinal Cord/embryology
  • Animals
  • Mosaicism
  • Phenotype
  • Genes, Recessive
  • Heterozygote
  • Microscopy, Electron
  • Gene Deletion
  • Zebrafish/anatomy & histology*
  • Axons/ultrastructure*
  • Axonal Transport
  • Brain/anatomy & histology*
  • Brain/embryology
  • Embryo, Nonmammalian
  • Mutation*
(all 20)
PubMed
1382472 Full text @ Neuron
Abstract
To determine the role of the floor plate (FP) in CNS development, I have used labeling techniques, including immunolabeling, to analyze cyclops mutant embryos, which lack the FP. Except for the anterior brain, the mutant phenotype is almost exclusively confined to the vicinity of the ventral CNS midline. In the midbrain, the number of ventral neurons is reduced and cell patterning is disturbed. In contrast, the neuronal arrangement in the spinal cord is almost normal, including in particular both primary and secondary motoneurons. Longitudinal axonal bundles are disorganized in both the brain and spinal cord. Laser ablating the FP in wild-type embryos locally phenocopies cyclops axonal disturbances, and transplanting wild-type FP precursor cells into mutants locally rescues the disturbances. These results demonstrate a significant role for the FP in pathfinding and fasciculation by axons in situ, especially during their longitudinal courses.
Genes / Markers
Figures
No images available
Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b16
    Deficiency
    1 - 1 of 1
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Fish
    Antibodies
    Orthology
    Engineered Foreign Genes
    No data available
    Mapping