PUBLICATION

Ectopic expression of Hoxa-1 in the zebrafish alters the fate of the mandibular arch neural crest and phenocopies a retinoic acid-induced phenotype

Authors
Alexandre, D., Clarke, J.D.W., Oxtoby, E., Yan, Y.L., Jowett, T., and Holder, N.
ID
ZDB-PUB-961014-27
Date
1996
Source
Development (Cambridge, England)   122(3): 735-746 (Journal)
Registered Authors
Alexandre, Daniel, Clarke, Jon, Holder, Nigel, Jowett, Trevor, Yan, Yi-Lin
Keywords
none
MeSH Terms
  • RNA, Messenger/genetics
  • Animals
  • Gene Expression Regulation, Developmental
  • Molecular Sequence Data
  • Phenotype
  • Homeodomain Proteins/metabolism
  • Amino Acid Sequence
  • Cloning, Molecular
  • Trans-Activators/physiology*
  • Mandible/embryology*
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Genes, Homeobox
  • Rhombencephalon/embryology
  • Base Sequence
  • Sequence Homology, Amino Acid
  • Neural Crest/cytology*
  • Tretinoin/pharmacology
  • Sequence Alignment
(all 19)
PubMed
8631251 Full text @ Development
Abstract
Considerable evidence has demonstrated that retinoic acid influences the formation of the primary body axis in vertebrates and that this may occur through the regulation of Hox gene expression. In this study, we show that the phenotype induced by exogenous retinoic acid in the zebrafish can also be generated by the overexpression of Hoxa-1 following injection of synthetic RNA into the fertilised egg. The isolation, sequence and expression pattern of the zebrafish Hoxa-1 gene is described. We show that exogenously applied retinoic acid causes the ectopic accumulation of Hoxa-1 message during gastrulation in the hypoblast in the head region. Overexpression of Hoxa-1 following injection of RNA causes abnormal growth of the anterior hindbrain, duplication of Mauthner neurons in rhombomere (r) 2 and fate changes of r2 mesenchymal and neurogenic neural crest. These results are discussed in terms of the role of Hoxa-1 in controlling anterior hindbrain patterning and the relationship between expression of Hoxa-1 and retinoic acid.
Genes / Markers
Figures
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Expression
Phenotype
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Mutations / Transgenics
No data available
Human Disease / Model
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Sequence Targeting Reagents
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Fish
Fish
WT
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Antibodies
Orthology
Gene Orthology
hoxb1b
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Engineered Foreign Genes
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Mapping
No data available