PUBLICATION

Ectopic expression of Hoxa-1 in the zebrafish alters the fate of the mandibular arch neural crest and phenocopies a retinoic acid-induced phenotype

Authors
Alexandre, D., Clarke, J.D.W., Oxtoby, E., Yan, Y.L., Jowett, T., and Holder, N.
ID
ZDB-PUB-961014-27
Date
1996
Source
Development (Cambridge, England)   122(3): 735-746 (Journal)
Registered Authors
Alexandre, Daniel, Clarke, Jon, Holder, Nigel, Jowett, Trevor, Yan, Yi-Lin
Keywords
none
MeSH Terms
  • Genes, Homeobox
  • Cloning, Molecular
  • Trans-Activators/physiology*
  • Mandible/embryology*
  • Base Sequence
  • Neural Crest/cytology*
  • Phenotype
  • Rhombencephalon/embryology
  • Homeodomain Proteins/metabolism
  • RNA, Messenger/genetics
  • Amino Acid Sequence
  • Molecular Sequence Data
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Gene Expression Regulation, Developmental
  • Animals
  • Tretinoin/pharmacology
  • Zebrafish/embryology*
  • Zebrafish/genetics*
(all 19)
PubMed
8631251 Full text @ Development
Abstract
Considerable evidence has demonstrated that retinoic acid influences the formation of the primary body axis in vertebrates and that this may occur through the regulation of Hox gene expression. In this study, we show that the phenotype induced by exogenous retinoic acid in the zebrafish can also be generated by the overexpression of Hoxa-1 following injection of synthetic RNA into the fertilised egg. The isolation, sequence and expression pattern of the zebrafish Hoxa-1 gene is described. We show that exogenously applied retinoic acid causes the ectopic accumulation of Hoxa-1 message during gastrulation in the hypoblast in the head region. Overexpression of Hoxa-1 following injection of RNA causes abnormal growth of the anterior hindbrain, duplication of Mauthner neurons in rhombomere (r) 2 and fate changes of r2 mesenchymal and neurogenic neural crest. These results are discussed in terms of the role of Hoxa-1 in controlling anterior hindbrain patterning and the relationship between expression of Hoxa-1 and retinoic acid.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
No data available
Fish
Fish
WT
1 - 1 of 1
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Antibodies
Name Type Antigen Genes Isotypes Host Organism
Ab1-engmonoclonalIgG1Mouse
Ab-3A10monoclonal
    IgG1Mouse
    1 - 2 of 2
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    Orthology
    Gene Orthology
    hoxb1b
    1 - 1 of 1
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    Engineered Foreign Genes
    No data available
    Mapping
    No data available