PUBLICATION

Ectopic expression of Hoxa-1 in the zebrafish alters the fate of the mandibular arch neural crest and phenocopies a retinoic acid-induced phenotype

Authors

Alexandre, D., Clarke, J.D.W., Oxtoby, E., Yan, Y.L., Jowett, T., and Holder, N.

ID

ZDB-PUB-961014-27

Date

1996

Source

Development (Cambridge, England) 122(3): 735-746 (Journal)

Registered Authors

Alexandre, Daniel, Clarke, Jon, Holder, Nigel, Jowett, Trevor, Yan, Yi-Lin

Keywords

none

MeSH Terms

Genes, Homeobox
Cloning, Molecular
Trans-Activators/physiology*
Mandible/embryology*
Base Sequence
Neural Crest/cytology*
Phenotype
Rhombencephalon/embryology
Homeodomain Proteins/metabolism
RNA, Messenger/genetics
Amino Acid Sequence
Molecular Sequence Data
Sequence Alignment
Sequence Homology, Amino Acid
Gene Expression Regulation, Developmental
Animals
Tretinoin/pharmacology
Zebrafish/embryology*
Zebrafish/genetics*

(all 19)

PubMed

8631251 Full text @ Development

Abstract

Considerable evidence has demonstrated that retinoic acid influences the formation of the primary body axis in vertebrates and that this may occur through the regulation of Hox gene expression. In this study, we show that the phenotype induced by exogenous retinoic acid in the zebrafish can also be generated by the overexpression of Hoxa-1 following injection of synthetic RNA into the fertilised egg. The isolation, sequence and expression pattern of the zebrafish Hoxa-1 gene is described. We show that exogenously applied retinoic acid causes the ectopic accumulation of Hoxa-1 message during gastrulation in the hypoblast in the head region. Overexpression of Hoxa-1 following injection of RNA causes abnormal growth of the anterior hindbrain, duplication of Mauthner neurons in rhombomere (r) 2 and fate changes of r2 mesenchymal and neurogenic neural crest. These results are discussed in terms of the role of Hoxa-1 in controlling anterior hindbrain patterning and the relationship between expression of Hoxa-1 and retinoic acid.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
dlx2a	WT	standard conditions	14-19 somites	neural crest	ISH	Fig. 10
egr2b	WT	standard conditions	20-25 somites	rhombomere 3	ISH	Fig. 6
egr2b	WT	standard conditions	20-25 somites	rhombomere 5	ISH	Fig. 6
hoxb1b	WT	standard conditions	Bud	axis	ISH	Fig. 3
hoxb1b	WT	standard conditions	20-25 somites	endoderm	ISH	Fig. 3
hoxb1b	WT	standard conditions	50%-epiboly	margin	ISH	Fig. 3
hoxb1b	WT	standard conditions	Shield to 90%-epiboly	margin	ISH	Fig. 3
hoxb1b	WT	standard conditions	20-25 somites	spinal cord neural tube	ISH	Fig. 3
hoxb1b	WT	standard conditions	Bud to 14-19 somites	spinal cord neural tube	ISH	Fig. 3
hoxb1b	WT	standard conditions	20-25 somites	tail bud	ISH	Fig. 3

1 - 10 of 10

Show

Phenotype

Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
WT

Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
Ab1-eng	monoclonal	en1a en1b en2a en2b	IgG1	Mouse
Ab-3A10	monoclonal		IgG1	Mouse

Orthology

Gene	Orthology
hoxb1b

Engineered Foreign Genes

Mapping

Alexandre et al., 1996 ZDB-PUB-961014-27 PMID:8631251

Ectopic expression of Hoxa-1 in the zebrafish alters the fate of the mandibular arch neural crest and phenocopies a retinoic acid-induced phenotype

Alexandre et al., 1996

ZDB-PUB-961014-27
PMID:8631251