PUBLICATION
Transmembrane Protein-184A Interacts with Syndecan-4 and Rab GTPases and Is Required to Maintain VE-Cadherin Levels
- Authors
- Altenburg, L.M., Wang, S.H., Ciabattoni, G.O., Kennedy, A., O'Toole, R.L., Farwell, S.L.N., Iovine, M.K., Lowe-Krentz, L.J.
- ID
- ZDB-PUB-250612-3
- Date
- 2025
- Source
- Cells 14: (Journal)
- Registered Authors
- Iovine, M. Kathryn
- Keywords
- TMEM184A, VE-cadherin, angiogenesis, endothelial cells, syndecan-4
- MeSH Terms
-
- Cattle
- Protein Binding
- Cadherins*/metabolism
- Neovascularization, Physiologic
- Zebrafish/metabolism
- Antigens, CD*/metabolism
- Humans
- Membrane Proteins*/metabolism
- Endothelial Cells/metabolism
- Syndecan-4*/metabolism
- Animals
- Cell Movement
- rab GTP-Binding Proteins*/metabolism
- PubMed
- 40498012 Full text @ Cells
Citation
Altenburg, L.M., Wang, S.H., Ciabattoni, G.O., Kennedy, A., O'Toole, R.L., Farwell, S.L.N., Iovine, M.K., Lowe-Krentz, L.J. (2025) Transmembrane Protein-184A Interacts with Syndecan-4 and Rab GTPases and Is Required to Maintain VE-Cadherin Levels. Cells. 14:.
Abstract
VE-cadherin (VE-cad) membrane stability and localization regulates adhesion formation and actin cytoskeleton dynamics in angiogenesis and vascular remodeling and requires the heparan sulfate proteoglycan (HSPG), Syndecan-4 (Sdc4). This study characterizes the interactions of the heparin receptor, Transmembrane protein-184A (TMEM184A), and Sdc4 in bovine aortic endothelial cells (BAOECs) and the regenerating Zebrafish (ZF) caudal fin and measures the effect of siRNA TMEM184A KD (siTMEM) and TMEM184A overexpression (TMEM OE) on VE-cad levels and localization in confluent and sub-confluent cultured BAOECs. Additionally, we examined the effect of siTMEM on key Rab GTPase trafficking regulators and migrating BAOECs in scratch wound healing assays. We demonstrated that TMEM184A and Sdc4 colocalize in BAOECs and that Sdc4 OE increases colocalization in an HS chain dependent manner, while both Tmem184a and Sdc4 cooperate synergistically in ZF fin angiogenic and tissue repair. We also showed that siTMEM decreases VE-cad membrane and cytoplasmic levels, while increasing scratch wound migration rates. However, TMEM OE cells show increased vesicle formation and VE-cad trafficking and membrane recovery. These findings characterize TMEM184A-Sdc4 cooperation in angiogenesis and indicate a dual function of TMEM184A in signaling and trafficking in vascular cells that promotes VE-cad recovery and membrane localization.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping