PUBLICATION
Tick salivary proteins metalloprotease and allergen-like p23 are associated with response to glycan α-Gal and mycobacterium infection
- Authors
- Vaz-Rodrigues, R., Mazuecos, L., Contreras, M., González-García, A., Rafael, M., Villar, M., de la Fuente, J.
- ID
- ZDB-PUB-250317-2
- Date
- 2025
- Source
- Scientific Reports 15: 88498849 (Journal)
- Registered Authors
- Keywords
- Allergy, Saliva, Tick, Tuberculosis, Zebrafish, α-Gal syndrome
- MeSH Terms
-
- Disease Models, Animal
- Salivary Proteins and Peptides/immunology
- Salivary Proteins and Peptides/metabolism
- Ticks/immunology
- Ticks/microbiology
- Food Hypersensitivity/immunology
- Food Hypersensitivity/metabolism
- Food Hypersensitivity/microbiology
- Humans
- Polysaccharides/immunology
- Polysaccharides/metabolism
- Animals
- Metalloproteases*/metabolism
- Allergens/immunology
- Mycobacterium Infections/immunology
- Zebrafish*/immunology
- PubMed
- 40087469 Full text @ Sci. Rep.
Citation
Vaz-Rodrigues, R., Mazuecos, L., Contreras, M., González-García, A., Rafael, M., Villar, M., de la Fuente, J. (2025) Tick salivary proteins metalloprotease and allergen-like p23 are associated with response to glycan α-Gal and mycobacterium infection. Scientific Reports. 15:88498849.
Abstract
The alpha-Gal syndrome (AGS) evolved as a catastrophic selection associated with anti-α-Gal IgM/IgG protective response against pathogen infection and tick-borne food allergy caused by IgE-type antibodies against this glycan present in glycoproteins and glycolipids from mammalian meat and derived products. The immune response to α-Gal is modulated by tick salivary proteins with and without α-Gal modifications in combination with tick saliva non-protein fraction. Herein, we characterized the role of tick salivary proteins, metalloprotease and allergen-like p23 in AGS and protection against tuberculosis in the AGS zebrafish animal model. Metalloprotease and p23 are involved in allergic reactions after mammalian meat consumption through upregulation of pro-inflammatory protein-coding genes prkdc, tlr2, tnfα and il1b. Challenge with Mycobacterium marinum activated Th1-mediated immune protective response with reduced pathogen infection, ameliorating Th2-associated allergic reactions associated with AGS. These results highlight molecular mechanisms modulated by tick proteins in response to α-Gal and provide insights to reduce AGS impact on human health.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping