PUBLICATION
The circadian clock orchestrates spermatogonial differentiation and fertilization by regulating retinoic acid signaling in vertebrates
- Authors
- Liu, T., He, W., Zhong, Z., Lu, C., Wu, L., Wang, Z., Smith, W.K., Shi, Q., Long, Q., Wang, H.
- ID
- ZDB-PUB-250307-31
- Date
- 2024
- Source
- National science review 12: nwae456nwae456 (Journal)
- Registered Authors
- Liu, Taole, Wang, Han, Zhong, Zhaomin
- Keywords
- circadian clock, fertilization, mice, retinoic acid, spermatogenesis, testis, zebrafish
- MeSH Terms
- none
- PubMed
- 40051524 Full text @ Natl Sci Rev
Citation
Liu, T., He, W., Zhong, Z., Lu, C., Wu, L., Wang, Z., Smith, W.K., Shi, Q., Long, Q., Wang, H. (2024) The circadian clock orchestrates spermatogonial differentiation and fertilization by regulating retinoic acid signaling in vertebrates. National science review. 12:nwae456nwae456.
Abstract
The circadian clock generates and maintains ∼24-hour oscillations in almost all organs. The testis, however, remains mysterious, without a clear understanding of its circadian functions. Our time-series transcriptome analysis reveals more than 1000 rhythmically expressed genes in the zebrafish and mouse testes, respectively. Canonical circadian clock genes are rhythmically expressed in Sertoli cells and regulate retinoic acid (RA) production, which is also evidenced by their co-expression with RA synthesis genes in single Sertoli cells. Genetic and pharmacological manipulations and temporal desynchronization revealed that the circadian clock-regulated RA signaling synchronizes spermatogonial differentiation via zbtb16a and promotes fertilization via izumo1 in zebrafish. Our findings indicate that the testicular circadian clock contributes to reproduction in a cell-specific manner through RA signaling, highlighting circadian roles in male fertility.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping