PUBLICATION
Protocol to perform ex vivo electroretinography on adult zebrafish
- Authors
- Simpson, S.L., Collery, R.F.
- ID
- ZDB-PUB-250118-7
- Date
- 2025
- Source
- STAR protocols 6: 103565103565 (Other)
- Registered Authors
- Collery, Ross
- Keywords
- Model Organisms, Neuroscience, Signal Transduction
- MeSH Terms
-
- Zebrafish*/physiology
- Software
- Retina*/physiology
- Electroretinography*/methods
- Animals
- PubMed
- 39823232 Full text @ STAR Protoc
Citation
Simpson, S.L., Collery, R.F. (2025) Protocol to perform ex vivo electroretinography on adult zebrafish. STAR protocols. 6:103565103565.
Abstract
Ex vivo electroretinography (ERG) provides insight into the health and functionality of retinal cells, the integrity of phototransduction, and the visual cycle and allows for the direct application of pharmaceuticals to the retinal tissues. Here, we present a protocol for performing ex vivo ERG on adult zebrafish. We describe steps for zebrafish tissue dissection, mounting tissues, and assembling and connecting cassettes. We then detail procedures for running the Diagnosys software and processing and analyzing the resulting data.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping