PUBLICATION
Knockout of rbm24a and rbm24b genes in zebrafish impairs skeletal and cardiac muscle integrity and function during development
- Authors
- Saquet, A., Ying, Z., Shi, D.L., Grifone, R.
- ID
- ZDB-PUB-240926-13
- Date
- 2024
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists : (Journal)
- Registered Authors
- Shi, De-Li
- Keywords
- RNA‐binding protein, Rbm24, heart, post‐transcriptional regulation, sarcomere, skeletal muscle, zebrafish
- MeSH Terms
-
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- RNA-Binding Proteins*/genetics
- RNA-Binding Proteins*/metabolism
- Muscle Development*/genetics
- Gene Expression Regulation, Developmental
- Heart*/embryology
- Muscle, Skeletal*/embryology
- Muscle, Skeletal*/metabolism
- Muscle, Skeletal*/physiology
- Gene Knockout Techniques
- Myocardium*/metabolism
- Zebrafish*/embryology
- Zebrafish*/genetics
- Animals
- PubMed
- 39323318 Full text @ Dev. Dyn.
Citation
Saquet, A., Ying, Z., Shi, D.L., Grifone, R. (2024) Knockout of rbm24a and rbm24b genes in zebrafish impairs skeletal and cardiac muscle integrity and function during development. Developmental Dynamics : an official publication of the American Association of Anatomists. :.
Abstract
Backgound Skeletal and cardiac muscles are contractile tissues whose development and function are dependent on genetic programs that must be precisely orchestrated in time and space. In addition to transcription factors, RNA-binding proteins tightly regulate gene expression by controlling the fate of RNA transcripts, thus specific proteins levels within the cell. Rbm24 has been identified as a key player of myogenesis and cardiomyogenesis in several vertebrates, by controlling various aspects of post-transcriptional regulation, including pre-mRNA alternative splicing and mRNA stabilization. In zebrafish, knockdown of rbm24a or rbm24b also causes skeletal and cardiac muscle phenotypes, but how their combined loss affects muscle integrity and function remains elusive.
Results By genome editing, we have generated rbm24a and rbm24b single mutants as well as double mutants. Structural analyses indicate that homozygous rbm24a and rbm24b double mutants exhibit severe somitic muscle and cardiac phenotypes, although rbm24b single mutants are obviously normal. We further show that the loss of rbm24a and rbm24b disrupts sarcomere organization, impairing functional contractility and motility of skeletal and cardiac muscles.
Conclusion The rbm24 mutant zebrafish represents a new genetic tool for in-depth studies of Rbm24-mediated post-transcriptional regulation of skeletal and cardiac muscle development, disease and regeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping