PUBLICATION

Mapping the cellular expression patterns of vascular endothelial growth factor aa and bb genes and their receptors in the adult zebrafish brain during constitutive and regenerative neurogenesis

Authors
Fernezelian, D., Pfitsch, S., Rastegar, S., Diotel, N.
ID
ZDB-PUB-240913-9
Date
2024
Source
Neural Development   19: 1717 (Journal)
Registered Authors
Diotel, Nicolas, Pfitsch, Sabrina, Rastegar, Sepand
Keywords
Brain, Flt1, Flt4, Kdr, Kdrl, Neural stem cells, Vegf, Zebrafish
MeSH Terms
  • Receptors, Vascular Endothelial Growth Factor/genetics
  • Receptors, Vascular Endothelial Growth Factor/metabolism
  • Neurogenesis*/physiology
  • Nerve Regeneration/physiology
  • Zebrafish Proteins*/genetics
  • Zebrafish Proteins*/metabolism
  • Vascular Endothelial Growth Factor B/genetics
  • Vascular Endothelial Growth Factor B/metabolism
  • Neural Stem Cells/metabolism
  • Animals
  • Brain*/metabolism
  • Vascular Endothelial Growth Factor A*/genetics
  • Vascular Endothelial Growth Factor A*/metabolism
  • Zebrafish*
(all 14)
PubMed
39267104 Full text @ Neural Dev.
Abstract
The complex interplay between vascular signaling and neurogenesis in the adult brain remains a subject of intense research. By exploiting the unique advantages of the zebrafish model, in particular the persistent activity of neural stem cells (NSCs) and the remarkable ability to repair brain lesions, we investigated the links between NSCs and cerebral blood vessels. In this study, we first examined the gene expression profiles of vascular endothelial growth factors aa and bb (vegfaa and vegfbb), under physiological and regenerative conditions. Employing fluorescence in situ hybridization combined with immunostaining and histology techniques, we demonstrated the widespread expression of vegfaa and vegfbb across the brain, and showed their presence in neurons, microglia/immune cells, endothelial cells and NSCs. At 1 day post-lesion (dpl), both vegfaa and vegfbb were up-regulated in neurons and microglia/peripheral immune cells (macrophages). Analysis of vegf receptors (vegfr) revealed high expression throughout the brain under homeostatic conditions, with vegfr predominantly expressed in neurons and NSCs and to a lower extent in microglia/immune cells and endothelial cells. These findings were further validated by Vegfr3 and Vegfr4 immunostainings, which showed significant expression in neurogenic radial glial cells.Following brain lesion (1 dpl), while vegfr gene expression remained stable, vegfr transcripts were detected in proliferative cells within the injured parenchyma. Collectively, our results provide a first overview of Vegf/Vegfr signaling in the brain and suggest important roles for Vegf in neurogenesis and regenerative processes.
Genes / Markers
Figures
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Expression
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Phenotype
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Mutations / Transgenics
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Human Disease / Model
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Sequence Targeting Reagents
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Fish
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Antibodies
Orthology
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Engineered Foreign Genes
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Mapping
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Citation
Fernezelian, D., Pfitsch, S., Rastegar, S., Diotel, N. (2024) Mapping the cellular expression patterns of vascular endothelial growth factor aa and bb genes and their receptors in the adult zebrafish brain during constitutive and regenerative neurogenesis. Neural Development. 19:1717.