PUBLICATION

Hox code responsible for the pattering of the anterior vertebrae in zebrafish

Authors
Maeno, A., Koita, R., Nakazawa, H., Fujii, R., Yamada, K., Oikawa, S., Tani, T., Ishizaka, M., Satoh, K., Ishizu, A., Sugawara, T., Adachi, U., Kikuchi, M., Iwanami, N., Matsuda, M., Kawamura, A.
ID
ZDB-PUB-240629-7
Date
2024
Source
Development (Cambridge, England)   151(14): (Journal)
Registered Authors
Kawamura, Akinori
Keywords
Hox gene, Micro-CT scan, Vertebral column, Vertebrate evolution, Zebrafish
MeSH Terms
  • Homeodomain Proteins*/genetics
  • Homeodomain Proteins*/metabolism
  • Oryzias/embryology
  • Oryzias/genetics
  • Animals
  • Spine*/embryology
  • Mice
  • Genes, Homeobox/genetics
  • Gene Expression Regulation, Developmental*
  • Zebrafish Proteins*/genetics
  • Zebrafish Proteins*/metabolism
  • Zebrafish*/embryology
  • Zebrafish*/genetics
  • Body Patterning*/genetics
(all 14)
PubMed
38940461 Full text @ Development
Abstract
The vertebral column is a characteristic structure of vertebrates. Genetic studies in mice have shown that Hox-mediated patterning plays a key role in specifying discrete anatomical regions of the vertebral column. Expression pattern analyses in several vertebrate embryos provided correlative evidence that the anterior boundaries of Hox expression coincide with distinct anatomical vertebrae. However, because functional analyses have been limited to mice, it remains unclear which Hox genes actually function in vertebral patterning in other vertebrates. In this study, various zebrafish hox mutants were generated for loss-of-function phenotypic analysis to functionally decipher the Hox code responsible for the zebrafish anterior vertebrae between the occipital and thoracic vertebrae. We found that hox genes in HoxB- and HoxC-related clusters participate in regulating the morphology of the zebrafish anterior vertebrae. In addition, medaka hoxc6a was found to be responsible for anterior vertebral identity as in zebrafish. Based on phenotypic similarities with Hoxc6 knockout mice, our results suggest that the Hox patterning system, including at least Hoxc6, may have been functionally established in the vertebral patterning of the common ancestor of ray-finned and lobe-finned fishes.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
No data available
Phenotype
Fish Conditions Stage Phenotype Figure
hoxb4asud131/sud131standard conditionsAdult
hoxb4asud131/sud131; hoxb5asud125/sud125standard conditionsAdult
hoxb4asud131/sud131; hoxb5asud125/sud125standard conditionsAdult
hoxb4asud144/sud144standard conditionsAdult
hoxb4asud144/sud144; hoxb5asud125/sud125standard conditionsAdult
hoxb4asud144/sud144; hoxb5asud125/sud125standard conditionsAdult
hoxb4asud144/sud144; hoxb5asud125/sud125standard conditionsAdult
hoxb5asud125/sud125standard conditionsAdult
hoxb5asud125/sud125standard conditionsAdult
hoxb5asud125/sud125; hoxb5bsud136/sud136standard conditionsAdult
1 - 10 of 37
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
sud122
    		Small Deletion
    sud125
      		Small Deletion
      sud126
        		Indel
        sud130
          		Small Deletion
          sud131
            		Small Deletion
            sud136
              		Indel
              sud137
                		Small Deletion
                sud138
                  		Small Deletion
                  sud144
                    		Small Deletion
                    1 - 9 of 9
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                    Human Disease / Model
                    No data available
                    Sequence Targeting Reagents
                    Target Reagent Reagent Type
                    hoxb4aCRISPR1-hoxb4aCRISPR
                    hoxb5aCRISPR3-hoxb5aCRISPR
                    hoxb5bCRISPR3-hoxb5bCRISPR
                    hoxb6aCRISPR1-hoxb6aCRISPR
                    hoxb6bCRISPR1-hoxb6bCRISPR
                    hoxc6aCRISPR1-hoxc6aCRISPR
                    hoxc6bCRISPR1-hoxc6bCRISPR
                    hoxc8aCRISPR1-hoxc8aCRISPR
                    1 - 8 of 8
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                    Fish
                    Fish
                    hoxb4asud131/sud131
                    hoxb4asud131/sud131; hoxb5asud125/sud125
                    hoxb4asud144/sud144
                    hoxb4asud144/sud144; hoxb5asud125/sud125
                    hoxb5asud125/sud125
                    hoxb5asud125/sud125; hoxb5bsud136/sud136
                    hoxb5bsud136/sud136
                    hoxb6asud130/sud130
                    hoxb6asud130/sud130; hoxb6bsud137/sud137
                    hoxb6bsud137/sud137
                    1 - 10 of 13
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                    Antibodies
                    No data available
                    Orthology
                    No data available
                    Engineered Foreign Genes
                    No data available
                    Mapping
                    No data available
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                    Citation
                    Maeno, A., Koita, R., Nakazawa, H., Fujii, R., Yamada, K., Oikawa, S., Tani, T., Ishizaka, M., Satoh, K., Ishizu, A., Sugawara, T., Adachi, U., Kikuchi, M., Iwanami, N., Matsuda, M., Kawamura, A. (2024) Hox code responsible for the pattering of the anterior vertebrae in zebrafish. Development (Cambridge, England). 151(14):.