PUBLICATION

scRNA-seq reveals the diversity of the developing cardiac cell lineage and molecular players in heart rhythm regulation

Authors
Abu Nahia, K., Sulej, A., Migdał, M., Ochocka, N., Ho, R., Kamińska, B., Zagorski, M., Winata, C.L.
ID
ZDB-PUB-240614-9
Date
2024
Source
iScience   27: 110083110083 (Journal)
Registered Authors
Abu Nahia, Karim, Sulej, Agata, Winata, Cecilia Lanny
Keywords
Embryology, Integrative aspects of cell biology, Model organism, Transcriptomics
Datasets
GEO:GSE234216
MeSH Terms
none
PubMed
38872974 Full text @ iScience
Abstract
We utilized scRNA-seq to delineate the diversity of cell types in the zebrafish heart. Transcriptome profiling of over 50,000 cells at 48 and 72 hpf defined at least 18 discrete cell lineages of the developing heart. Utilizing well-established gene signatures, we identified a population of cells likely to be the primary pacemaker and characterized the transcriptome profile defining this critical cell type. Two previously uncharacterized genes, atp1b3b and colec10, were found to be enriched in the sinoatrial cardiomyocytes. CRISPR/Cas9-mediated knockout of these two genes significantly reduced heart rate, implicating their role in cardiac development and conduction. Additionally, we describe other cardiac cell lineages, including the endothelial and neural cells, providing their expression profiles as a resource. Our results established a detailed atlas of the developing heart, providing valuable insights into cellular and molecular mechanisms, and pinpointed potential new players in heart rhythm regulation.
Genes / Markers
Figures
Figure Gallery (5 images)
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
atp1b3btwu34Tgstandard conditionsProtruding-mouthISH
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Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
md3TgTransgenic Insertion
    s883TgTransgenic Insertion
      sqet31EtTransgenic Insertion
        sqet33mi59BEtTransgenic Insertion
          twu34TgTransgenic Insertion
            1 - 5 of 5
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            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            Fish
            Antibodies
            No data available
            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            EGFPEFGEGFP
            mRFPEFGmRFP
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            Mapping
            No data available