PUBLICATION

A Robust Closed-Tube Method for Resolving tp53M214K Genotypes

Authors
Silvius, K.M., Kendall, G.C.
ID
ZDB-PUB-240223-1
Date
2024
Source
Zebrafish   21(3): 250-254 (Journal)
Registered Authors
Kendall, Genevieve
Keywords
HRMA, genotyping, tp53, tp53M214K zebrafish mutant, wild-type spike-in
MeSH Terms
  • Animals
  • Mutation
  • Genotyping Techniques*/methods
  • Zebrafish Proteins/genetics
  • Genotype
  • Tumor Suppressor Protein p53*/genetics
  • Zebrafish*/genetics
PubMed
38386541 Full text @ Zebrafish
Abstract
The tp53M214K zebrafish mutant is a versatile platform with which to model a diverse spectrum of human diseases. However, currently available genotyping methods for this mutant require lengthy hands-on processes such as restriction digests and outsourced Sanger sequencing. To address this deficiency, we leveraged high-resolution melting analysis technology in conjunction with a parallel, in-tandem wild-type spike-in approach to develop a robust genotyping protocol capable of discriminating tp53M214K zygosity. In this study, we describe our method in detail. We anticipate that our genotyping protocol will benefit researchers utilizing the tp53M214K zebrafish mutant by offering reliable results with a shorter turnaround time, lower personnel involvement, and higher throughput than traditional methods, thereby decreasing the burden of genotyping and maximizing research efficiency.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping