PUBLICATION

RhoA GEF Mcf2lb regulates rosette integrity during collective cell migration

Authors
Olson, H.M., Maxfield, A., Calistri, N.L., Heiser, L.M., Qian, W., Knaut, H., Nechiporuk, A.V.
ID
ZDB-PUB-240103-24
Date
2024
Source
Development (Cambridge, England)   151(1): (Journal)
Registered Authors
Knaut, Holger, Nechiporuk, Alex, Olson, Hannah
Keywords
Apical constriction, Cell migration, Rosette morphogenesis, Sensory development, Zebrafish lateral line
Datasets
GEO:GSE229567
MeSH Terms
  • Cell Movement/genetics
  • Lateral Line System*
  • Signal Transduction/physiology
  • Zebrafish Proteins/metabolism
  • Morphogenesis/physiology
  • Animals
  • Zebrafish*/metabolism
(all 7)
PubMed
38165177 Full text @ Development
Abstract
Multicellular rosettes are transient epithelial structures that serve as important cellular intermediates in the formation of diverse organs. Using the zebrafish posterior lateral line primordium (pLLP) as a model system, we investigated the role of the RhoA GEF Mcf2lb in rosette morphogenesis. The pLLP is a group of ∼150 cells that migrates along the zebrafish trunk and is organized into epithelial rosettes; these are deposited along the trunk and will differentiate into sensory organs called neuromasts (NMs). Using single-cell RNA-sequencing and whole-mount in situ hybridization, we showed that mcf2lb is expressed in the pLLP during migration. Live imaging and subsequent 3D analysis of mcf2lb mutant pLLP cells showed disrupted apical constriction and subsequent rosette organization. This resulted in an excess number of deposited NMs along the trunk of the zebrafish. Cell polarity markers ZO-1 and Par-3 were apically localized, indicating that pLLP cells are properly polarized. In contrast, RhoA activity, as well as signaling components downstream of RhoA, Rock2a and non-muscle Myosin II, were diminished apically. Thus, Mcf2lb-dependent RhoA activation maintains the integrity of epithelial rosettes.
Genes / Markers
Figures
Figure Gallery (9 images)
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
ackr3bsk115Tg/sk115Tg; zf106Tg/zf106Tgstandard conditionsPrim-15RTPCR
arhgef4sk115Tg/sk115Tg; zf106Tg/zf106Tgstandard conditionsPrim-15ISH
EGFPmcf2lbnl25/nl25; sk115Tg/sk115Tg; zf106Tg/zf106Tgstandard conditionsProtruding-mouthIFL
EGFPmcf2lbnl25/nl25; sk115Tg/sk115Tg; zf106Tg/zf106Tgstandard conditionsProtruding-mouthIFL
EGFPmcf2lbnl25/nl25; sk122Tg/sk122Tg; sk85Tg/sk85Tgstandard conditionsPrim-25IFL
EGFPmcf2lbnl25/nl25; zf106Tg/zf106Tgstandard conditionsPrim-15IFL
EGFPmcf2lbnl25/nl25; zf106Tg/zf106Tgstandard conditionsPrim-15IFL
EGFPmcf2lbnl26/nl26; sk115Tg/sk115Tg; zf106Tg/zf106Tgstandard conditionsProtruding-mouthIFL
EGFPmcf2lbnl26/nl26; sk122Tg/sk122Tg; sk85Tg/sk85Tgstandard conditionsPrim-25IFL
EGFPmcf2lbnl26/nl26; zf106Tg/zf106Tgstandard conditionsPrim-15IFL
1 - 10 of 22
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Phenotype
Fish Conditions Stage Phenotype Figure
mcf2lbnl25/nl25; sk115Tg/sk115Tg; zf106Tg/zf106Tgstandard conditionsProtruding-mouth
mcf2lbnl25/nl25; sk115Tg/sk115Tg; zf106Tg/zf106Tgstandard conditionsProtruding-mouth
mcf2lbnl25/nl25; sk115Tg/sk115Tg; zf106Tg/zf106Tgstandard conditionsProtruding-mouth
mcf2lbnl25/nl25; sk122Tg/sk122Tg; sk85Tg/sk85Tgstandard conditionsPrim-25
mcf2lbnl25/nl25; sk122Tg/sk122Tg; sk85Tg/sk85Tgstandard conditionsPrim-25
mcf2lbnl25/nl25; zf106Tg/zf106Tgstandard conditionsPrim-15
mcf2lbnl25/nl25; zf106Tg/zf106Tgstandard conditionsPrim-15
mcf2lbnl25/nl25; zf106Tg/zf106Tgstandard conditionsPrim-15
mcf2lbnl25/nl25; zf106Tg/zf106Tgstandard conditionsHigh-pec
mcf2lbnl25/nl25; zf106Tg/zf106Tgstandard conditionsHigh-pec
1 - 10 of 22
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
nl25
    		Complex
    nl26
      		Complex
      sk85TgTransgenic Insertion
        sk115TgTransgenic Insertion
          sk122TgTransgenic Insertion
            zf106TgTransgenic Insertion
              zf668TgTransgenic Insertion
                1 - 7 of 7
                Show
                Human Disease / Model
                No data available
                Sequence Targeting Reagents
                Target Reagent Reagent Type
                mcf2lbCRISPR1-mcf2lbCRISPR
                mcf2lbCRISPR2-mcf2lbCRISPR
                mcf2lbCRISPR3-mcf2lbCRISPR
                1 - 3 of 3
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                Fish
                Fish
                mcf2lbnl25/nl25; sk115Tg/sk115Tg; zf106Tg/zf106Tg
                mcf2lbnl25/nl25; sk115Tg/sk115Tg; zf106Tg/zf106Tg
                mcf2lbnl25/nl25; sk122Tg/sk122Tg; sk85Tg/sk85Tg
                mcf2lbnl25/nl25; zf106Tg/zf106Tg
                mcf2lbnl26/nl26; sk115Tg/sk115Tg; zf106Tg/zf106Tg
                mcf2lbnl26/nl26; sk122Tg/sk122Tg; sk85Tg/sk85Tg
                mcf2lbnl26/nl26; zf106Tg/zf106Tg
                sk115Tg/sk115Tg; zf106Tg/zf106Tg
                sk122Tg/sk122Tg; sk85Tg/sk85Tg
                zf106Tg/zf106Tg
                1 - 10 of 10
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                Antibodies
                Orthology
                No data available
                Engineered Foreign Genes
                Marker Marker Type Name
                mScarletEFGmScarlet
                CFPEFGCFP
                DsRedEFGDsRed
                EGFPEFGEGFP
                mCherryEFGmCherry
                sfGFPEFGsfGFP
                1 - 6 of 6
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                Mapping
                No data available
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                Citation
                Olson, H.M., Maxfield, A., Calistri, N.L., Heiser, L.M., Qian, W., Knaut, H., Nechiporuk, A.V. (2024) RhoA GEF Mcf2lb regulates rosette integrity during collective cell migration. Development (Cambridge, England). 151(1):.