PUBLICATION

nkx2.3 is responsible for posterior pharyngeal cartilage formation by inhibiting Fgf signaling

Authors
Yang, S., Xu, X., Yin, Z., Liu, Y., Wang, H., Guo, J., Wang, F., Bao, Y., Zhang, T., Sun, S.
ID
ZDB-PUB-231201-6
Date
2023
Source
Heliyon   9: e21915e21915 (Journal)
Registered Authors
Yang, Shuyan
Keywords
Differentiation, Fgf signaling, Posterior pharyngeal cartilages, Zebrafish, nkx2.3
MeSH Terms
none
PubMed
38034615 Full text @ Heliyon
Abstract
Nkx2.3, a transcription factor, plays important roles in various developmental processes. However, the mechanisms underlying nkx2.3's regulation of pouch and pharyngeal arch development in zebrafish remain unclear. In this study, we demonstrated that knockdown or knockout of nkx2.3 resulted in the absence of posterior ceratobranchial cartilages in zebrafish. The absence of posterior pharyngeal cartilages is a consequence of the compromised proliferation and differentiation and survival of cranial neural crest cells (CNCCs). Notably, we found that nkx2.3 was not involved in endoderm pouch formation. Additionally, our findings suggested that nkx2.3 negatively regulated Fibroblast growth factor (Fgf) signaling, as overexpression of fgf8 could mimic the phenotype observed in nkx2.3 morphants, suppressing CNCC differentiation. Moreover, inhibiting Fgf signaling restored the abnormalities in posterior cartilages induced by nkx2.3 knockdown. These findings establish the essential role of nkx2.3 in the development of posterior ceratobranchial cartilages through the inhibition of fgf8.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b1193TgTransgenic Insertion
    bj2
      Complex
      s870TgTransgenic Insertion
        y1TgTransgenic Insertion
          zdf1
            Point Mutation
            1 - 5 of 5
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            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            Target Reagent Reagent Type
            nkx2.3CRISPR1-nkx2.3CRISPR
            nkx2.3CRISPR2-nkx2.3CRISPR
            nkx2.3MO1-nkx2.3MRPHLNO
            nkx2.3MO2-nkx2.3MRPHLNO
            1 - 4 of 4
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            Fish
            Antibodies
            Name Type Antigen Genes Isotypes Host Organism
            Ab1-GFPmonoclonalMouse
            Ab36-h3monoclonal
              IgGRabbit
              zn-5monoclonalIgG1Mouse
              1 - 3 of 3
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              Orthology
              No data available
              Engineered Foreign Genes
              Marker Marker Type Name
              EGFPEFGEGFP
              GFPEFGGFP
              1 - 2 of 2
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              Mapping
              No data available