PUBLICATION

The sinusoidal hematopoietic niche is formed by Jam1a via Notch signaling in the zebrafish kidney

Authors: Kondo, M., Kimura, K., Kobayashi-Sun, J., Yamamori, S., Taniguchi, M., Traver, D., Kobayashi, I.
ID: ZDB-PUB-230501-42
Date: 2023
Source: iScience 26: 106508106508 (Journal)
Registered Authors: Kobayashi, Isao, Traver, David
Keywords: Animal physiology, Biological sciences, Molecular physiology, Physiology
MeSH Terms: none
PubMed: 37123221 Full text @ iScience

Abstract

The zebrafish is a unique model to understand hematopoietic niches as hematopoietic stem/progenitor cells are maintained in the kidney. However, little is known about which cell types in the kidney play a role in hematopoietic niches. Here, we demonstrate that the sinusoidal endothelium is an essential and conserved niche component in the zebrafish kidney. Histological analysis revealed that runx1:mCherry⁺ hematopoietic cells were predominantly detected in the dorsolateral region of the kidney where sinusoids are highly developed. Loss of Junctional adhesion molecule 1a (Jam1a), which is expressed in both sinusoidal endothelial cells and hematopoietic cells, resulted in a remarkable reduction in sinusoids and a defect in hematopoietic niches. We found that Jam1a regulates jagged-1a expression in vascular endothelial cells to form a sinusoidal structure in the kidney. Collectively, these data suggest that sinusoids are formed by Jam1a via endothelial Notch signaling to provide hematopoietic niches in the zebrafish kidney.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
angpt1	f11r.1^sd43/sd43; sd24Tg	standard conditions	Adult	kidney vascular endothelium	RTPCR	Figure 6
Cerulean	cz2010Tg; sd24Tg	standard conditions	Adult	kidney sinusoidal blood vessel endothelium	IHC	Figure 2
Cerulean	f11r.1^sd43/sd43; kca3Tg; sd24Tg; ubs4Tg	standard conditions	Adult	kidney sinusoidal blood vessel endothelium	IHC	Figure 8
Cerulean	f11r.1^sd43/sd43; sd24Tg	standard conditions	Adult	kidney sinusoidal blood vessel endothelium	IHC	Figure 6
Cerulean	f11r.1^sd43/sd43; sd24Tg	standard conditions	Adult	kidney vascular endothelium	IHC	Figure 6
Cerulean	f11r.1^sd43/sd43; sd24Tg; ubs4Tg	standard conditions	Adult	kidney sinusoidal blood vessel endothelium	IHC	Figure 8
Cerulean	jag1a^kz4/kz4; sd24Tg	standard conditions	Adult	kidney sinusoidal blood vessel endothelium	IHC	Figure 7
Cerulean	sd24Tg	standard conditions	Adult	dorsal aorta vascular endothelium	IHC	Figure 2
Cerulean	sd24Tg	standard conditions	Adult	kidney sinusoidal blood vessel endothelium	IHC	Figure 2
Cerulean	sd24Tg	standard conditions	Adult	kidney sinusoidal blood vessel endothelium	IHC	Figure 7

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
f11r.1^sd43/sd43	standard conditions	Prim-5	dorsal aorta has fewer parts of type hematopoietic stem cell, abnormal	Figure S3
f11r.1^sd43/sd43	standard conditions	Prim-5	whole organism f11r.1 expression absent, abnormal	Figure S3
f11r.1^sd43/sd43	standard conditions	Adult	collecting duct f11r.1 expression absent, abnormal	Figure S3
f11r.1^sd43/sd43	standard conditions	Adult	kidney regulation of hematopoietic progenitor cell differentiation decreased process quality, abnormal	Figure 5
f11r.1^sd43/sd43	standard conditions	Adult	renal tubule f11r.1 expression absent, abnormal	Figure S3
f11r.1^sd43/sd43; kca3Tg; sd24Tg; ubs4Tg	standard conditions	Adult	kidney has number of sinusoidal blood vessel endothelium, ameliorated	Figure 8
f11r.1^sd43/sd43; kca3Tg; sd24Tg; ubs4Tg	standard conditions	Adult	kidney sinusoidal blood vessel endothelium her2 expression amount, ameliorated	Figure 8
f11r.1^sd43/sd43; kca3Tg; sd24Tg; ubs4Tg	standard conditions	Adult	kidney sinusoidal blood vessel endothelium her6 expression increased amount, abnormal	Figure 8
f11r.1^sd43/sd43; kca3Tg; sd24Tg; ubs4Tg	standard conditions	Adult	kidney sinusoidal blood vessel endothelium hey1 expression increased amount, abnormal	Figure 8
f11r.1^sd43/sd43; kca3Tg; sd24Tg; ubs4Tg	standard conditions	Adult	kidney sinusoidal blood vessel endothelium hey2 expression decreased amount, abnormal	Figure 8

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
cz2010Tg	Tg(Mmu.Runx1:NLS-mCherry)	Transgenic Insertion
kca3Tg	Tg(5xUAS-E1B:6xMYC-notch1a)	Transgenic Insertion
kz4		Small Deletion	jag1a
la3Tg	Tg(-20.7gata2:EGFP)	Transgenic Insertion
s898Tg	Tg(kdrl:Cre)	Transgenic Insertion
sd24Tg	Tg(kdrl:Cerulean)	Transgenic Insertion
sd27Tg	Tg(actb2:LOXP-BFP-LOXP-DsRed)	Transgenic Insertion
sd43		Small Deletion	f11r.1
ubs4Tg	Tg(fli1:GAL4FF)	Transgenic Insertion

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Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
f11r.1	CRISPR1-f11r.1	CRISPR
jag1a	CRISPR1-jag1a	CRISPR

Fish

Fish
AB
cz2010Tg; la3Tg
cz2010Tg; sd24Tg
f11r.1^sd43/sd43
f11r.1^sd43/sd43; cz2010Tg; la3Tg
f11r.1^sd43/sd43; kca3Tg; sd24Tg; ubs4Tg
f11r.1^sd43/sd43; kca3Tg; ubs4Tg
f11r.1^sd43/sd43; s898Tg; sd27Tg
f11r.1^sd43/sd43; sd24Tg
f11r.1^sd43/sd43; sd24Tg; ubs4Tg

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Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
Ab1-gapdh	monoclonal	gapdh		Mouse

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
BFP	EFG	BFP
Cerulean	EFG	Cerulean
Cre	EFG	Cre
DsRed	EFG	DsRed
EGFP	EFG	EGFP
GAL4FF	EFG	GAL4FF
mCherry	EFG	mCherry

Mapping

Kondo et al., 2023 ZDB-PUB-230501-42 PMID:37123221

The sinusoidal hematopoietic niche is formed by Jam1a via Notch signaling in the zebrafish kidney

Kondo et al., 2023

ZDB-PUB-230501-42
PMID:37123221