PUBLICATION

Alcam-a and Pdgfr-α are essential for the development of sclerotome-derived stromal cells that support hematopoiesis

Authors
Murayama, E., Vivier, C., Schmidt, A., Herbomel, P.
ID
ZDB-PUB-230302-41
Date
2023
Source
Nature communications   14: 11711171 (Journal)
Registered Authors
Herbomel, Philippe, Murayama, Emi, Schmidt, Anne, Vivier, Catherine
Keywords
none
MeSH Terms
  • Receptor, Platelet-Derived Growth Factor alpha*
  • Hematopoiesis
  • Zebrafish
  • Mammals
  • Stromal Cells
  • Activated-Leukocyte Cell Adhesion Molecule*
  • Animals
  • Hematopoietic Stem Cells
  • Receptor Protein-Tyrosine Kinases
(all 9)
PubMed
36859431 Full text @ Nat. Commun.
Abstract
Mesenchymal stromal cells are essential components of hematopoietic stem and progenitor cell (HSPC) niches, regulating HSPC proliferation and fates. Their developmental origins are largely unknown. In zebrafish, we previously found that the stromal cells of the caudal hematopoietic tissue (CHT), a niche functionally homologous to the mammalian fetal liver, arise from the ventral part of caudal somites. We have now found that this ventral domain is the sclerotome, and that two markers of mammalian mesenchymal stem/stromal cells, Alcam and Pdgfr-α, are distinctively expressed there and instrumental for the emergence and migration of stromal cell progenitors, which in turn conditions the proper assembly of the vascular component of the CHT niche. Furthermore, we find that trunk somites are similarly dependent on Alcam and Pdgfr-α to produce mesenchymal cells that foster HSPC emergence from the aorta. Thus the sclerotome contributes essential stromal cells for each of the key steps of developmental hematopoiesis.
Genes / Markers
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
i150TgTransgenic Insertion
    ia5TgTransgenic Insertion
      mu271TgTransgenic Insertion
        rk8TgTransgenic Insertion
          s896TgTransgenic Insertion
            sq11TgTransgenic Insertion
              sqet37EtTransgenic Insertion
                zf73TgTransgenic Insertion
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                  Engineered Foreign Genes
                  Marker Marker Type Name
                  DsRedEFGDsRed
                  EGFPEFGEGFP
                  GAL4EFGGAL4
                  GFPEFGGFP
                  KaedeEFGKaede
                  mCherryEFGmCherry
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