PUBLICATION

GTP-Binding Protein 1-Like (GTPBP1l) Regulates Vascular Patterning during Zebrafish Development

Authors
Lo, Y.H., Huang, Y.S., Chang, Y.C., Hung, P.Y., Wang, W.D., Liu, W., Urade, R., Wen, Z.H., Wu, C.Y.
ID
ZDB-PUB-221224-8
Date
2022
Source
Biomedicines   10(12): (Journal)
Registered Authors
Keywords
CVP (caudal vein plexus), ISV (intersegmental vessel), gtpbp1l, vascular development, zebrafish
MeSH Terms
none
PubMed
36551964 Full text @ Biomedicines
Abstract
Genetic regulation of vascular patterning is not fully understood. Here, we report a novel gene, gtpbp1l (GTP-binding protein 1-like), that regulates vascular development in zebrafish. Amino acid sequence comparison and a phylogenetic study showed that gtpbp1l is conserved in vertebrates. Gtpbp1l mRNA is expressed in the vasculature during embryogenesis. Knockdown of gtpbp1l by morpholino impairs the patterning of the intersegmental vessel (ISV) and caudal vein plexus (CVP), indicating the role of gtpbp1l in vasculature. Further apoptosis assays and transgenic fish tests suggested that vascular defects in gtpbp1l morphants are not due to cell death but are likely caused by the impairment of migration and proliferation. Moreover, the altered expression of vessel markers is consistent with the vascular defects in gtpbp1l morphants. Finally, we revealed that gtpbp1l is regulated by VEGF/notch and BMP signaling. Collectively, these findings showed that gtpbp1l plays a critical role in vascular patterning during zebrafish development.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ci5TgTransgenic Insertion
    la116TgTransgenic Insertion
      sd2TgTransgenic Insertion
        y1TgTransgenic Insertion
          y7TgTransgenic Insertion
            1 - 5 of 5
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            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            Target Reagent Reagent Type
            gtpbp1lMO1-gtpbp1lMRPHLNO
            gtpbp1lMO2-gtpbp1lMRPHLNO
            1 - 2 of 2
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            Fish
            Antibodies
            No data available
            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            DsRedEFGDsRed
            EGFPEFGEGFP
            GFPEFGGFP
            mCherryEFGmCherry
            1 - 4 of 4
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            Mapping
            No data available