PUBLICATION

Csf1rb regulates definitive hematopoiesis in zebrafish

Authors
Dai, Y., Wu, S., Cao, C., Xue, R., Luo, X., Wen, Z., Xu, J.
ID
ZDB-PUB-220726-30
Date
2022
Source
Development (Cambridge, England)   149(16): (Journal)
Registered Authors
Wen, Zilong, Xue, Rongtao, Xu, Jin
Keywords
Hematopoiesis, Hematopoietic stem and progenitor cells, Zebrafish, csf1r
MeSH Terms
  • Hematopoietic Stem Cells/physiology
  • Mammals
  • Zebrafish*/genetics
  • Hematopoiesis/genetics
  • Zebrafish Proteins*/genetics
  • Animals
  • Cell Differentiation/physiology
(all 7)
PubMed
35876681 Full text @ Development
Abstract
In vertebrates, hematopoietic stem and progenitor cells (HSPCs) are capable of self-renewal and continuously replenishing all mature blood lineages throughout life. However, the molecular signaling regulating the maintenance and expansion of HSPCs remains not fully understood. Colony-stimulating factor 1 receptor (Csf1r) is believed to be the primary regulator for myeloid lineage but not HSPC development. Here, we show a surprising role of Csf1rb, a zebrafish homolog of mammalian CSF1R, in preserving the HSPCs pool by maintaining the proliferation of HSPCs. The deficiency of csf1rb leads to a reduction in both HSPCs and their differentiated progenies, including myeloid, lymphoid, and erythroid cells at early developmental stages. Likewise, the absence of csf1rb conferred similar defects upon HSPCs and leukocytes in adulthood. Furthermore, adult hematopoietic cells from csf1rb mutants failed to repopulate immuno-deficient zebrafish. Interestingly, loss-of-function and gain-of-function assays suggested that the canonical ligands for CSF1R in zebrafish, including Csf1a, Csf1b, and Il34, were unlikely to be the ligand of Csf1rb. Thus, our data indicate a previously unappreciated role of CSF1R in maintaining HSPCs, which is independent of known ligands.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
csf1rbhkz50/hkz50standard conditionsProtruding-mouth
csf1rbhkz50/hkz50standard conditionsProtruding-mouth
csf1rbhkz50/hkz50standard conditionsDay 5
csf1rbhkz50/hkz50standard conditionsDay 5 to Day 6
csf1rbhkz50/hkz50standard conditionsDay 5 to Day 6
csf1rbhkz50/hkz50; hkz011tTg/hkz011tTg; hkz04tTg/hkz04tTgstandard conditionsAdult
csf1rbhkz50/hkz50; ncv6Tg/ncv6Tgultraviolet lightPrim-15 to Long-pec
csf1rbhkz50/hkz50; ncv6Tg/ncv6Tgultraviolet lightProtruding-mouth
csf1rbhkz50/hkz50; zf169Tg/zf169Tgstandard conditionsProtruding-mouth
csf1rbhkz50/hkz50; zf169Tg/zf169Tgstandard conditionsProtruding-mouth
1 - 10 of 11
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
cq22TgTransgenic Insertion
    hg1
      Point Mutation
      hkz04tTgTransgenic Insertion
        hkz09tTgTransgenic Insertion
          hkz10
            Small Deletion
            hkz11
              Small Deletion
              hkz011tTgTransgenic Insertion
                hkz016tTgTransgenic Insertion
                  hkz017tTgTransgenic Insertion
                    hkz047TgTransgenic Insertion
                      1 - 10 of 19
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Target Reagent Reagent Type
                      csf1rbCRISPR7-csf1rbCRISPR
                      csf1rbCRISPR8-csf1rbCRISPR
                      1 - 2 of 2
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                      Fish
                      Antibodies
                      Name Type Antigen Genes Isotypes Host Organism
                      Ab3-gata1apolyclonal
                        IgGRabbit
                        Ab3-lcp1polyclonalRabbit
                        1 - 2 of 2
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                        Orthology
                        No data available
                        Engineered Foreign Genes
                        Marker Marker Type Name
                        DsRedxEFGDsRedx
                        EGFPEFGEGFP
                        EosEFGEos
                        GFPEFGGFP
                        KaedeEFGKaede
                        1 - 5 of 5
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                        Mapping
                        No data available