PUBLICATION

Peripheral nerve development in zebrafish requires muscle patterning by tcf15/paraxis

Authors
Limbach, L.E., Penick, R.L., Casseday, R.S., Hyland, M.A., Pontillo, E.A., Ayele, A.N., Pitts, K.M., Ackerman, S.D., Harty, B.L., Herbert, A.L., Monk, K.R., Petersen, S.C.
ID
ZDB-PUB-220714-5
Date
2022
Source
Developmental Biology   490: 37-49 (Journal)
Registered Authors
Petersen, Sarah C.
Keywords
Lateral line nerve, Myelin, Paraxial mesoderm, Schwann cells, Zebrafish
MeSH Terms
  • Basic Helix-Loop-Helix Transcription Factors
  • Muscles
  • Zebrafish*/genetics
  • Peripheral Nervous System
  • Animals
  • Peripheral Nerves*
  • Axons/physiology
(all 7)
PubMed
35820658 Full text @ Dev. Biol.
Abstract
The vertebrate peripheral nervous system (PNS) is an intricate network that conveys sensory and motor information throughout the body. During development, extracellular cues direct the migration of axons and glia through peripheral tissues. Currently, the suite of molecules that govern PNS axon-glial patterning is incompletely understood. To elucidate factors that are critical for peripheral nerve development, we characterized the novel zebrafish mutant, stl159, that exhibits abnormalities in PNS patterning. In these mutants, motor and sensory nerves that develop adjacent to axial muscle fail to extend normally, and neuromasts in the posterior lateral line system, as well as neural crest-derived melanocytes, are incorrectly positioned. The stl159 genetic lesion lies in the basic helix-loop-helix (bHLH) transcription factor tcf15, which has been previously implicated in proper development of axial muscles. We find that targeted loss of tcf15 via CRISPR-Cas9 genome editing results in the PNS patterning abnormalities observed in stl159 mutants. Because tcf15 is expressed in developing muscle prior to nerve extension, rather than in neurons or glia, we predict that tcf15 non-cell-autonomously promotes peripheral nerve patterning in zebrafish through regulation of extracellular patterning cues. Our work underscores the importance of muscle-derived factors in PNS development.
Genes / Markers
Figures
No images available
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
sl3TgTransgenic Insertion
    stl159
      Point Mutation
      zf148TgTransgenic Insertion
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        tcf15CRISPR1-tcf15CRISPR
        tcf15CRISPR2-tcf15CRISPR
        tcf15CRISPR3-tcf15CRISPR
        1 - 3 of 3
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        Fish
        Antibodies
        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        DsRedEFGDsRed
        EGFPEFGEGFP
        1 - 2 of 2
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        Mapping
        No data available