PUBLICATION

mafba and mafbb differentially regulate lymphatic endothelial cell migration in topographically distinct manners

Authors
Arnold, H., Panara, V., Hußmann, M., Filipek-Gorniok, B., Skoczylas, R., Ranefall, P., Gloger, M., Allalou, A., Hogan, B.M., Schulte-Merker, S., Koltowska, K.
ID
ZDB-PUB-220623-8
Date
2022
Source
Cell Reports   39: 110982 (Journal)
Registered Authors
Hogan, Ben M., Koltowska, Kaska, Schulte-Merker, Stefan
Keywords
CP: Cell biology, CP: Developmental biology, Vegfc, Vegfr3, cell migration, lymphangiogenesis, lymphatics, mafba, mafbb
MeSH Terms
  • Zebrafish*
  • Signal Transduction
  • Morphogenesis
  • Endothelial Cells
  • Cell Movement
  • Animals
  • Lymphatic Vessels*
  • Lymphangiogenesis
(all 8)
PubMed
35732122 Full text @ Cell Rep.
Abstract
Lymphangiogenesis, formation of lymphatic vessels from pre-existing vessels, is a dynamic process that requires cell migration. Regardless of location, migrating lymphatic endothelial cell (LEC) progenitors probe their surroundings to form the lymphatic network. Lymphatic-development regulation requires the transcription factor MAFB in different species. Zebrafish Mafba, expressed in LEC progenitors, is essential for their migration in the trunk. However, the transcriptional mechanism that orchestrates LEC migration in different lymphatic endothelial beds remains elusive. Here, we uncover topographically different requirements of the two paralogs, Mafba and Mafbb, for LEC migration. Both mafba and mafbb are necessary for facial lymphatic development, but mafbb is dispensable for trunk lymphatic development. On the molecular level, we demonstrate a regulatory network where Vegfc-Vegfd-SoxF-Mafba-Mafbb is essential in facial lymphangiogenesis. We identify that mafba and mafbb tune the directionality of LEC migration and vessel morphogenesis that is ultimately necessary for lymphatic function.
Genes / Markers
Figures
Figure Gallery (4 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
mafbauq4bh/uq4bh; mafbbuq47bh/uq47bh; nim5Tg; y7Tgstandard conditionsDay 5
mafbauq4bh/uq4bh; mafbbuq47bh/uq47bh; nz101Tgstandard conditionsDay 5
mafbauq4bh/uq4bh; mafbbuq47bh/uq47bh; nz101Tgstandard conditionsDay 5
mafbauq4bh/uq4bh; mafbbuq47bh/uq47bh; nz101Tgstandard conditionsDay 5
mafbauq4bh/uq4bh; mafbbuq47bh/uq47bh; nz101Tgstandard conditionsDay 5
mafbauq4bh/uq4bh; mafbbuq47bh/uq47bh; nz101Tg; y7Tgstandard conditionsDay 5
mafbauq4bh/uq4bh; mafbbuq47bh/uq47bh; nz101Tg; y7Tgstandard conditionsDay 5
mafbauq4bh/uq4bh; mafbbuq47bh/uq47bh; nz101Tg; y7Tgstandard conditionsDay 5
mafbauq4bh/uq4bh; mafbbuq47bh/uq47bh; nz101Tg; y7Tgstandard conditionsDay 5
mafbauq4bh/uq4bh; mafbbuq47bh/uq47bh; uu1kkTgstandard conditionsPrim-25 to High-pec
1 - 10 of 25
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
hu7135TgTransgenic Insertion
    nim5TgTransgenic Insertion
      nz101TgTransgenic Insertion
        s916TgTransgenic Insertion
          uq4bh
            Point Mutation
            uq37bhTgTransgenic Insertion
              uq47bh
                Small Deletion
                uu1kkTgTransgenic Insertion
                  uu2kkTgTransgenic Insertion
                    y7TgTransgenic Insertion
                      1 - 10 of 10
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Target Reagent Reagent Type
                      mafbbCRISPR5-mafbbCRISPR
                      sox7MO1-sox7MRPHLNO
                      sox18MO1-sox18MRPHLNO
                      vegfcMO1-vegfcMRPHLNO
                      vegfcMO3-vegfcMRPHLNO
                      vegfdMO1-vegfdMRPHLNO
                      1 - 6 of 6
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                      Fish
                      Antibodies
                      Name Type Antigen Genes Isotypes Host Organism
                      Ab5-prox1polyclonalIgGRabbit
                      Ab16-GFPpolyclonalIgYChicken
                      1 - 2 of 2
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                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      CitrineEFGCitrine
                      DsRedEFGDsRed
                      EGFPEFGEGFP
                      KALTA4EFGKALTA4
                      mCherryEFGmCherry
                      TagRFPEFGTagRFP
                      VenusEFGVenus
                      1 - 7 of 7
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                      Mapping
                      No data available