PUBLICATION

Probiotics Enhance Bone Growth and Rescue BMP Inhibition: New Transgenic Zebrafish Lines to Study Bone Health

Authors
Sojan, J.M., Raman, R., Muller, M., Carnevali, O., Renn, J.
ID
ZDB-PUB-220515-9
Date
2022
Source
International Journal of Molecular Sciences   23(9): (Journal)
Registered Authors
Carnevali, Oliana, Muller, Marc
Keywords
BMP inhibitors, Danio rerio, bone growth, bone matrix, mineralization, probiotics, transgenic lines, zebrafish
MeSH Terms
  • Osteoblasts/metabolism
  • Animals, Genetically Modified
  • Animals
  • Larva/genetics
  • Probiotics*/pharmacology
  • Bone Density
  • Zebrafish*/genetics
  • Bone Development
(all 8)
PubMed
35563140 Full text @ Int. J. Mol. Sci.
Abstract
Zebrafish larvae, especially gene-specific mutants and transgenic lines, are increasingly used to study vertebrate skeletal development and human pathologies such as osteoporosis, osteopetrosis and osteoarthritis. Probiotics have been recognized in recent years as a prophylactic treatment for various bone health issues in humans. Here, we present two new zebrafish transgenic lines containing the coding sequences for fluorescent proteins inserted into the endogenous genes for sp7 and col10a1a with larvae displaying fluorescence in developing osteoblasts and the bone extracellular matrix (mineralized or non-mineralized), respectively. Furthermore, we use these transgenic lines to show that exposure to two different probiotics, Bacillus subtilis and Lactococcus lactis, leads to an increase in osteoblast formation and bone matrix growth and mineralization. Gene expression analysis revealed the effect of the probiotics, particularly Bacillus subtilis, in modulating several skeletal development genes, such as runx2, sp7, spp1 and col10a1a, further supporting their ability to improve bone health. Bacillus subtilis was the more potent probiotic able to significantly reverse the inhibition of bone matrix formation when larvae were exposed to a BMP inhibitor (LDN212854).
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ulg001TgTransgenic Insertion
    ulg071TgTransgenic Insertion
    ulg072TgTransgenic Insertion
    1 - 3 of 3
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    col10a1aCRISPR3-col10a1aCRISPR
    entpd5aMO1-entpd5aMRPHLNO
    sp7CRISPR2-sp7CRISPR
    tp53MO5-tp53MRPHLNO
    1 - 4 of 4
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    Fish
    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    GFPEFGGFP
    mCherryEFGmCherry
    1 - 2 of 2
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    Mapping
    No data available