PUBLICATION

A new transgenic reporter line reveals expression of protocadherin 9 at a cellular level within the zebrafish central nervous system

Authors
Habicher, J., Manuel, R., Pedroni, A., Ferebee, C., Ampatzis, K., Boije, H.
ID
ZDB-PUB-220419-8
Date
2022
Source
Gene expression patterns : GEP   44: 119246 (Journal)
Registered Authors
Boije, Henrik, Habicher, Judith, Manuel, Remy
Keywords
none
MeSH Terms
  • Protocadherins*
  • Motor Neurons/metabolism
  • Animals, Genetically Modified
  • Animals
  • Zebrafish*/genetics
  • Zebrafish*/metabolism
  • Central Nervous System/metabolism
  • Cadherins/genetics
  • Cadherins/metabolism
  • Spinal Cord/metabolism
(all 10)
PubMed
35427788 Full text @ Gene Expr. Patterns
Abstract
The wiring of neuronal networks is far from understood. One outstanding question is how neurons of different types link up to form subnetworks within the greater context. Cadherins have been suggested to create an inclusion code where interconnected neurons express the same subtypes. Here, we have used a CRISPR/Cas9 knock-in approach to generate a transgenic zebrafish reporter line for protocadherin 9 (pcdh9), which is predominantly expressed within the central nervous system. Expression of eGFP was detected in subsets of neurons in the cerebellum, retina and spinal cord, in both larvae and juveniles. A closer characterization of the spinal locomotor network revealed that a portion of distinct classes of both excitatory and inhibitory interneurons, as well as motor neurons, expressed pcdh9. This transgenic line could thus be used to test the cadherin network hypothesis, through electrophysiological characterization of eGFP positive cells, to show if these are synaptically connected and form a discrete network within the spinal cord.
Genes / Markers
Figures
Figure Gallery (4 images)
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b1222TgTransgenic Insertion
    ia6TgTransgenic Insertion
      nkuasrfp1aTgTransgenic Insertion
        nns6TgTransgenic Insertion
          nns61TgTransgenic Insertion
            uu233TgTransgenic Insertion
            1 - 6 of 6
            Show
            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            Target Reagent Reagent Type
            pcdh9CRISPR2-pcdh9CRISPR
            pcdh9CRISPR3-pcdh9CRISPR
            1 - 2 of 2
            Show
            Fish
            Fish
            pcdh9uu233Tg (AB)
            pcdh9uu233Tg; b1222Tg; nkuasrfp1aTg
            pcdh9uu233Tg; ia6Tg
            pcdh9uu233Tg; nkuasrfp1aTg; nns6Tg
            pcdh9uu233Tg; nkuasrfp1aTg; nns61Tg
            1 - 5 of 5
            Show
            Antibodies
            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            DsRedEFGDsRed
            EGFPEFGEGFP
            GAL4EFGGAL4
            RFPEFGRFP
            1 - 4 of 4
            Show
            Mapping
            No data available
            Your Input Welcome
            We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
            Citation
            Habicher, J., Manuel, R., Pedroni, A., Ferebee, C., Ampatzis, K., Boije, H. (2022) A new transgenic reporter line reveals expression of protocadherin 9 at a cellular level within the zebrafish central nervous system. Gene expression patterns : GEP. 44:119246.